Video: Dab tsi yog substrate rau beta galactosidase?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
β-galactosides suav nrog cov carbohydrates galactose cov qhov twg cov nyiaj glycosidic nyob saum toj no galactose cov molecule. Cov khoom sib txawv ntawm β-galactosidases suav nrog ganglioside GM1, lactosylceramides, lactose, thiab ntau yam glycoproteins.
Los ntawm qhov ntawd, vim li cas Onpg siv los ua cov substrate rau B galactosidase?
ortho-Nitrophenyl- β - galactoside hmoov ( ONPG ) yog xim xim thiab spectrophotometric txheej rau kev nrhiav pom ntawm β - galactosidase kev ua. β - Galactosidase yuav tsum tau siv rau lactose, yog li siv cov xim tsim tau tuaj yeem yog siv raws li kev ntsuas tus nqi enzymatic.
Ib yam nkaus, beta galactosidase zom cov lactose li cas? β - Galactosidase muaj peb yam kev ua enzymatic (Daim duab 1). Ua ntej, nws tuaj yeem tshem cov disaccharide cov lactose tsim cov piam thaj thiab galactose, uas tuaj yeem nkag mus rau glycolysis. Qhov thib ob, cov enzyme tuaj yeem cuam tshuam cov transgalactosylation ntawm cov lactose rau allolactose, thiab, thib peb, allolactose tuaj yeem ua rau cov monosaccharides.
Ua qhov no hauv kev txiav txim siab, kev tshuaj ntsuam beta galactosidase yog dab tsi?
Keeb kwm yav dhau β - Galactosidase yog cim los ntawm lacZ noob ntawm lac operon hauv E. coli. Nws yog cov protein loj (120 kDa, 1024 amino acids) uas tsim tetramer. Vim li no, ONPG/ β -Kev kev ntsuam xyuas yog hu ua "Miller" kev ntsuam xyuas , thiab tus txheej txheem txheem ntawm β -Gal kev ua haujlwm yog "Miller Unit".
Puas muaj beta galactosidase nyob qhov twg?
GLB1 noob muab cov lus qhia rau tsim cov enzyme hu ua beta - galactosidase (β- galactosidase ). Qhov enzyme no yog nyob hauv lysosomes, uas yog cov chav nyob hauv cov cell uas zom thiab rov ua dua ntau hom sib txawv.
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