Cov txheej txheem:
Video: Koj ntsuas kev ua enzyme hauv ib lub chaw kuaj mob li cas?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2024-01-18 09:28
Kev ntsuam xyuas enzyme
- Enzyme kev ntsuam xyuas yog kuaj txoj kev rau ntsuas kev ua haujlwm enzymatic .
- Qhov ntau los yog qhov concentration ntawm ib qho enzyme tuaj yeem nthuav tawm hauv cov nyiaj molar, xws li nrog rau lwm yam tshuaj, lossis hais txog kev ua nyob rau hauv enzyme chav nyob.
- Kev ua haujlwm enzyme = moles ntawm substrate hloov pauv rau ib chav sijhawm = tus nqi × cov tshuaj tiv thaiv ntim.
Hauv qhov no, spectrophotometer siv li cas los ntsuas kev ua enzyme?
Thaum lub sij hawm a spectrophotometric kev soj ntsuam , tus neeg teb xov tooj ua raws txoj kev ntawm enzyme tshuaj tiv thaiv los ntawm ntsuas cov kev hloov hauv kev siv ntawm lub teeb absorbed los yog tawg los ntawm cov tshuaj tiv thaiv. Qee zaum, ntau tshaj ib lub wavelength yuav tsum muaj siv los tsim cov cim zoo rau xam cov kev ua haujlwm ntawm enzyme.
Tsis tas li ntawd, koj ntsuas kev ua lactase li cas? Kev ntsuas Enzyme Kev ua Ntxiv 10 µL ntawm lactase extract rau cov tshuaj tiv thaiv B raj, sib tov los ntawm vortexing thiab cia cov tshuaj tiv thaiv mus rau 1 min ntawm chav tsev kub. Thaum 1 min tau tom qab, ntxiv 500 μL ntawm 1 M sodium carbonate rau ob leeg hlab inhibit qhov kev lactase enzyme los ntawm kev nce pH, yog li txiav tawm cov tshuaj tiv thaiv.
Xav txog qhov no, vim li cas peb thiaj ntsuas kev ua haujlwm enzyme?
Kev ua haujlwm enzyme yog a ntsuas ntau npaum li cas enzyme muaj nyob rau hauv cov tshuaj tiv thaiv. Muaj ob txoj hauv kev ntsuas kev ua haujlwm enzyme : saib xyuas qhov ploj ntawm cov substrate lossis cov khoom zoo li. Enzyme kev tshuaj ntsuam kuj tseem tuaj yeem nthuav qhia cov ntaub ntawv hais txog cov substrates thiab inhibitors uas tuaj yeem cuam tshuam rau enzyme.
Dab tsi yog qhov cuam tshuam rau kev ua haujlwm enzyme?
Muaj ntau yam cuam tshuam rau tus nqi uas cov tshuaj tiv thaiv enzymatic txuas mus - kub , pH, cov enzyme concentration , substrate concentration , thiab muaj cov inhibitors lossis activators.
Pom zoo:
Tus pas ntsuas ntsuas ntsuas ntsuas ntsuas ntsuas yog dab tsi?
Qhov kev ntsuas no siv cov khoom paub ntawm lub ntiajteb txawj nqus los txiav txim siab tias tus neeg yuav siv sijhawm ntev npaum li cas los teb rau qhov poob ntawm ib yam khoom los ntawm kev ntsuas deb npaum li cas cov khoom tuaj yeem ntog ua ntej raug ntes
Ob qhov kev ntsuam xyuas ntshav twg yog qhov tseem ceeb tshaj plaws hauv kev ntsuas kev ntsuas lub raum ua haujlwm?
Urea thiab Creatinine yog ob yam tshuaj hauv cov ntshav uas tau tshuaj xyuas thaum ntsuas kev ua haujlwm ntawm lub raum. Lawv raug tshem tawm ntawm lub cev los ntawm lub raum los ntawm kev pom thiab tso tawm
Koj puas tuaj yeem siv lub ntsuas kub kub los ntsuas koj qhov ntsuas kub?
Hmoov zoo, koj tuaj yeem siv lub ntsuas kub ntawm cov nqaij los xyuas koj lub cev kub. Nws tsis yog qhov tseeb raws li lub ntsuas cua qhov ncauj, tab sis nws tuaj yeem pab koj taug qab koj lub cev kub li cas
Qhov ntsuas qhov ntsuas qhov ntsuas qhov ntsuas kub npaum li cas?
Cov qhab nias thiab txhais lus CAS muaj 21 yam khoom nrog 4 qhov kev xaiv txhua tus (uas tau qhab nia 0,1,2,3). CAS tus qhab nia tsuas yog qhov sib npaug ntawm cov qhab nia khoom. Yog li, cov qhab nia ntawm CAS tuaj yeem nyob ntawm 0 txog 63. Qhov qhab nia siab dua txhais tau tias tus neeg koom nrog muaj cov tsos mob npau taws ntau dua
Koj yuav ntsuas lub ntsuas ntsuas ntsev li cas?
Cov kauj ruam Calibration Ua raws li koj lub khw cov lus qhia. Yaug cov electrode hauv deionised lossis distilled dej? Dip electrode rau hauv lub khob tshiab ntawm cov tshuaj haum tsim nyog. Qhib lub ntsuas ntsuas salinity thiab kho qhov nyeem ntawv raws li cov lus qhia. Tua lub ntsuas dej ntsev thiab yaug dua hauv dej distilled