Video: Puas yog neomycin ib g418?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
g418 ua (Geneticin) yog aminoglycoside tshuaj tua kab mob zoo ib yam hauv tus qauv rau gentamicin B1. G418 yog ib qho analog ntawm neomycin sulfate, thiab muaj cov txheej txheem zoo ib yam li neomycin . g418 ua feem ntau siv hauv kev tshawb fawb tshawb fawb los xaiv cov noob caj noob ces (feem ntau siv KanMX xaiv tus cim).
Ua kom pom qhov no, puas yog neomycin tib yam li g418?
g418 ua tseem hu ua geneticin, yog ib qho dav-spectrum tshuaj tua kab mob uas yuav xaiv mammalian hlwb qhia txog neomycin protein (encoded los ntawm neomycin noob). Neomycin yuav xaiv cov kab mob uas qhia cov ib yam protein, tab sis feem ntau tus txhawb nqa rau Neo nyob rau hauv mammalian qhia plasmids tsis siv tau rau cov kab mob.
Qhov thib ob, kev kho g418 yog dab tsi? g418 ua tseem hu ua G-418, g418 ua sulfate lossis Geneticin yog cov tshuaj tua kab mob aminoglycoside tsim los ntawm Micromonospora rhodorangea. G418 blocks polypeptide synthesis los ntawm inhibiting cov kauj ruam elongation nyob rau hauv ob leeg prokaryotic thiab eukaryotic hlwb.
Hauv qhov no, puas yog neomycin zoo ib yam li kanamycin?
Feem ntau, neomycin yog siv hauv kev sim ntawm cov hlwb prokaryotic, thaum G418 yog siv hauv kev sim eukaryotic. Kanamycin yog ib qho tshuaj tua kab mob aminoglycoside uas ua haujlwm los ntawm inhibiting translocation ntawm ribosome uas ua rau mistranslation.
Dab tsi yog noob neomycin tsis kam?
Neomycin tsis kam yog conferred los ntawm ib qho ntawm ob aminoglycoside phosphotransferase noob . Neomycin khi rau 30S subunit ntawm ribosome thiab txwv tsis pub txhais cov protein los ntawm mRNA.
Pom zoo:
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