Cov txheej txheem:
Video: Koj suav cov cell hauv Haemocytometer li cas?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Rau suav cov cell siv a hemocytometer , ntxiv 15-20Μl ntawm ntawm tes suspension nruab nrab ntawm cov hemocytometer thiab npog iav siv P-20 Pipetman. Lub hom phiaj yog kom muaj kwv yees li 100-200 hlwb /kub. Suav tus naj npawb ntawm hlwb nyob rau hauv tag nrho plaub sab squares faib los ntawm plaub (tus naj npawb ntawm hlwb / square).
Tib neeg tseem nug, koj suav cov cell nyob li cas?
Txhawm rau xam tus naj npawb ntawm cov cell siv tau/ml:
- Siv qhov suav nruab nrab ntawm cov xov tooj ntawm txhua qhov teeb tsa ntawm 16 lub kaum plaub fab.
- Muab 10,000 (104).
- Muab faib los ntawm 5 los kho rau 1: 5 dilution los ntawm Trypan Blue ntxiv.
Tsis tas li ntawd, vim li cas OD siv los ntsuas tus lej xov tooj? Kev kho qhov muag ceev ( OD ) ntawm kab lis kev cai yog ntsuas los kwv yees kev loj hlob thiab kev ua haujlwm metabolic ntawm cov hlwb . Kev kho qhov muag ceev yog kev ua haujlwm logarithmic thiab ua rau nce ntxiv tus lej ntawm lub teeb nqus dej los ntawm ib qho txhais tau tias kev siv lub teeb dhau los ntawm cov qauv tau ploj zuj zus 10 zaug!
Kuj Paub, koj suav cov cell hauv Neubauer chav li cas?
Qhov chaw Neubauer chamber nyob rau theem tsom iav. Siv lub hom phiaj 10X, tsom ob qho tib si rau ntawm kab sib chaws thiab cov ntawm tes cov khoom. Raws li 10X yog qhov tsim nyog rau WBC suav , suav tag nrho cov naj npawb ntawm hlwb pom nyob rau hauv 4 lub kaum loj squares.
Vim li cas peb thiaj siv Haemocytometer?
Lub cuab yeej siv rau kev txiav txim tus naj npawb ntawm cov cell ntawm ib chav tsev ntim ntawm kev ncua yog hu ua chav suav. Nws yog tam sim no siv suav lwm hom xov tooj ntawm tes thiab lwm yam kab mob me me ib yam. Cov hemocytometer tau tsim los ntawm Louis-Charles Malassez.
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