Dab tsi yog isoelectric point hauv chemistry?
Dab tsi yog isoelectric point hauv chemistry?

Video: Dab tsi yog isoelectric point hauv chemistry?

Video: Dab tsi yog isoelectric point hauv chemistry?
Video: How To Calculate The Isoelectric Point of Amino Acids and Zwitterions 2024, Lub Xya hli ntuj
Anonim

Cov isoelectric taw tes (pI, pH (I), IEP), yog pH uas lub molecule nqa tsis tau hluav taws xob net los yog hluav taws xob nruab nrab nyob rau hauv qhov kev txheeb cais. Tus qauv nomenclature los sawv cev rau isoelectric point yog pH (I), txawm hais tias pI feem ntau pom, thiab siv rau hauv kab lus no rau brevity.

Ntxiv mus, lub ntsiab lus ntawm isoelectric point yog dab tsi?

Isoelectronic taw tes , pI The isoelectronic taw tes lub isoionic taw tes yog pH uas cov amino acid tsis tsiv mus nyob rau hauv hluav taws xob teb. Qhov no txhais tau tias nws yog pH uas cov amino acid yog nruab nrab, piv txwv li daim ntawv zwitterion yog dominant. ib table pKa thiab pI qhov tseem ceeb tuaj yeem pom nyob rau nplooj ntawv tom ntej.

Tsis tas li ntawd, yuav ua li cas tshwm sim ntawm lub isoelectric point? Cov isoelectric taw tes ( pI ) yog pH ntawm kev daws teeb meem uas tus nqi ntawm cov protein ua xoom. Ib yam li ntawd, ntawm qhov kev daws teeb meem pH uas yog qis dua pI ib , qhov chaw ntawm cov protein yog feem ntau zoo them, thiab repulsion ntawm cov proteins tshwm sim.

Ib tug kuj yuav nug, dab tsi yog txhais los ntawm isoelectric point nws qhov tseem ceeb yog dab tsi?

Isoelectric Point . Qhov isoelectric point (pI) yog cov pH nqi ntawm qhov cov molecule nqa tsis tau hluav taws xob. Cov lub tswvyim tshwj xeeb tseem ceeb rau zwitterionic molecules xws li amino acids, peptides, thiab cov protein. Qhov no tuaj yeem txuas ntxiv rau lub ntsiab lus ntawm pI ntawm peptides thiab cov protein.

Vim li cas cov protein tsis zoo them?

A protein ntau yog tsim los ntawm kev sib xyaw ntawm cov acidic, yooj yim (polar) thiab nruab nrab (tsis-polar) amino acids. Yog li, yog tias protein ntau muaj ntau ntawm cov amino acids yooj yim nws yuav zoo them nyiaj thiab yog tias nws muaj ntau ntawm cov kua qaub amino acids nws yuav yog tsis zoo nqi.

Pom zoo: