Video: Tus immunoblot qhia dab tsi rau koj?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Western Blotting (tseem hu ua immunoblotting ) yog txheej txheem siv rau kev tshuaj xyuas cov tib neeg cov protein hauv cov protein sib xyaw (piv txwv li cell lysate). Cov protein nyob ntawm no immunoblot tom qab ntawd nkag tau yooj yim rau cov tshuaj tiv thaiv kab mob txhawm rau txhawm rau txheeb xyuas. Cov tshuaj tiv thaiv kab mob tau siv los tshuaj xyuas cov hom phiaj ntawm cov protein sab hnub poob ( immunoblot ).
Xav txog qhov no, immunoblotting siv rau dab tsi?
Western blotting (protein ntau immunoblotting ) yog txheej txheem tshuaj xyuas siv rau txheeb xyuas thiab nrhiav cov protein tshwj xeeb hauv cov qauv ntawm cov ntaub so ntswg homogenate lossis rho tawm, raws li lawv lub peev xwm los khi rau cov tshuaj tiv thaiv tshwj xeeb.
Tom qab ntawd, lo lus nug yog, vim li cas thiaj li txhaj tshuaj tiv thaiv kab mob 2 kauj ruam? Keeb kwm, kev tshawb nrhiav txheej txheem tau ua hauv ob cov kauj ruam vim tias tus txheeb ze yooj yim ntawm kev tsim cov tshuaj tiv thaiv thawj thiab theem ob hauv cais txheej txheem . Qhov no muab cov kws tshawb fawb thiab cov tuam txhab muaj txiaj ntsig zoo hais txog kev yoog raws, thiab ntxiv kev nthuav dav kauj ruam mus nrhiav kom tau txheej txheem.
Kuj paub, kev tshuaj ntsuam immunoblot yog dab tsi?
Paub daws teeb. Immunoblotting (sab hnub poob blotting) yog qhov nrawm thiab nkag siab kev ntsuam xyuas rau kev kuaj pom thiab tus yam ntxwv ntawm cov protein uas ua haujlwm los ntawm kev siv qhov tshwj xeeb uas muaj nyob hauv antigen-antibody paub.
Vim li cas cov tshuaj tiv thaiv kab mob feem ntau siv los tshuaj xyuas cov protein?
Thawj tshuaj tiv thaiv kab mob Thawj tshuaj tiv thaiv tau tsim tawm thaum tus tswv tsev hom lossis kab lis kev cai tiv thaiv kab mob ntawm tes raug nthuav tawm rau protein ntau ntawm kev txaus siab (lossis ib feem ntawm nws). Feem ntau , qhov no yog ib feem ntawm kev tiv thaiv kab mob, qhov ntawm no lawv tau sau thiab siv raws li rhiab thiab tshwj xeeb nrhiav kom tau cov cuab yeej uas khi cov protein ntau ncaj qha
Pom zoo:
Cov hauv paus hauv siab dab tsi qhia pom tus neeg cawm neeg nyob qhov twg los thawb kom CPR nrawm rau ntawm tus menyuam mos?
Compression Point thiab Qhov tob Muab ob ntiv tes rau ntawm qhov chaw ntawm tus menyuam mos lub hauv siab, ncaj qha rau ntawm lub sternum thiab me ntsis hauv qab ntawm lub txiv mis. Qhov tob ntawm compression rau cov me nyuam mos yog hais txog 1½ ntiv tes (los yog 1/3 lub anteriorposterior txoj kab uas hla ntawm lub hauv siab)
Thaum twg koj yuav tsum hu rau koj tus thawj saib xyuas txog koj tus neeg noj zaub mov hauv Oregon?
Tos tsawg kawg 24 teev tom qab ntuav los yog raws plab tau tso tseg ua ntej rov mus ua haujlwm. Qhia rau koj tus thawj saib xyuas yog tias koj muaj cov tsos mob no. Yog tus thawj tswj hwm muaj lus nug, nws lossis nws tuaj yeem hu rau Lub Chaw Saib Xyuas Kev Noj Qab Haus Huv Hauv Nroog. Tsis txhob kov cov khoom noj uas muaj kab mob, hlawv, txiav lossis mob ntawm koj txhais tes
Dab tsi yog cov lus nug uas koj yuav tsum nug koj tus kheej thaum koj ntsuas koj tus kheej rau kev ntxhov siab?
Hauv qab no pom peb lo lus nug Hanna qhia koj tus kheej thaum koj muaj kev ntxhov siab txhawm rau ua kom koj tus kheej rov qab los. 'Qhov kev nyuab siab no puas yog kuv lub siab lossis lwm tus?' 'Kuv puas tuaj yeem pab tau?' 'Kuv yuav tsum tsim kom muaj ciam teb dab tsi?'
Puas yog txhua yam koj qhia rau koj tus kws kho mob tsis pub lwm tus paub?
A: Koj tus kws kho mob yuav khaws cov ntsiab lus ntawm qhov koj tham txog ntiag tug, lossis tsis pub lwm tus paub. Tib lub sijhawm uas koj tus kws kho mob tsis tuaj yeem hwm koj tus kheej yog thaum ib tus neeg ua phem rau koj lossis koj yuav ua phem rau koj tus kheej lossis lwm tus
Kuv yuav ua li cas los ua tus kws qhia Red Cross lifeguard tus kws qhia?
Yam yuavtsum tau kawm uantej: Txhawm rau koom nrog Txoj Haujlwm Pabcuam Kev Nyab Xeeb, koj yuav tsum: Muaj hnub nyoog tsawg kawg yog 17 xyoo nyob rau hnub kawg ntawm tus xibfwb qhia. Muaj los yog: Daim ntawv pov thawj Red Cross tam sim no rau Kev Pab Cuam Tshuam/Kev Pab Thawj/CPR/AED