Video: Qhov sib txheeb ntawm pKa thiab pI qhov tseem ceeb hauv cov protein yog dab tsi?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Cov pI ib ntawm a protein ntau tau txiav txim siab los ntawm kev sib sau pH (thiab yog li ntawd pKa ua ) ntawm txhua qhov amino acid hauv protein ntau saw. Txhua cov amino acid nws muaj nws tus kheej pKa (thiab pI ), tab sis tuaj yeem sib txawv raws li pes tsawg lwm cov amino acids nyob ib puag ncig koj lub hom phiaj amino acid.
Tom qab ntawd, ib tus kuj tseem tuaj yeem nug, pKa zoo ib yam li isoelectric point?
Cov isoelectric taw tes yog qhov nruab nrab ntawm 2 pKa ua qhov tseem ceeb uas muaj cov molecule nruab nrab raws li ib qho ntawm nws hom tsiaj sib npaug.
Qhov thib ob, tus nqi pK yog dab tsi? Siv ceev Kev ntsuas ntawm lub zog ntawm cov kua qaub ntawm cov lej logarithmic. Cov pK tus nqi yog muab los ntawm lub cav10(1/K. Ka), qhov twg Ka yog cov kua qaub sib cais tas mus li. pK qhov tseem ceeb feem ntau siv los sib piv qhov ua tau zoo ntawm cov kua qaub sib txawv. Los ntawm: pK tus nqi Hauv phau ntawv txhais lus ntawm Chemistry »
Qhov thib ob, pI qhia koj li cas txog cov protein?
Isoelectric taw tes , tseem hu ua pI ib ntawm protein ntau , yog pH uas tus nqi nqa ntawm protein ntau yog xoom. Isoelectric taw tes ( pI ib ): PH uas tus nqi them rau ntawm protein ntau yog xoom. Rau a protein ntau nrog ntau cov amino acids yooj yim, cov p ces ua siab, thaum rau acidic protein ntau cov p ces ua qis dua.
PKa sawv ntsug rau dab tsi?
Cov ntsiab lus tseem ceeb: pKa ua Txhais Cov pKa ua tus nqi yog ib txoj hauv kev siv los qhia qhov muaj zog ntawm cov kua qaub. pKa ua yog qhov tsis zoo ntawm cov kua qaub sib cais tsis tu ncua lossis Ka tus nqi. Ib qho qis dua pKa ua tus nqi qhia tau tias muaj zog acid.
Pom zoo:
Dab tsi yog qhov sib txawv tseem ceeb ntawm txheeb cais thiab tsis txheeb ze txheeb cais?
Kev ntsuas tus lej, uas qhov kev xav tshwj xeeb tau ua txog cov pej xeem qhov ntsuas tau hu ua kev ntsuas ntsuas. Kev ntsuas tus lej siv hauv qhov xwm txheej uas tsis yog ntsuas qhov sib txawv yog hu ua nonparametric test. Hauv kev ntsuas parametric, qhov ntsuas ntsuas yog ua raws kev faib khoom
Dab tsi yog qhov sib txawv thiab qhov zoo sib xws ntawm cov khoom sib tsoo thiab qhov kov zoo?
Dab tsi yog qhov sib txawv thiab qhov zoo sib xws ntawm 'crude' kov thiab 'nplua' kov qhov hnov? Nyuaj kov yog hais txog kev nkag siab los ntawm kev txhawb nqa ntawm cov neeg txais khoom ntawm qhov tsis nkag siab nrog cov teb loj. Hauv kev sib piv, kev kov zoo yog hais txog cov neeg txais khoom ntawm kev nkag siab siab nrog cov chaw txais me me
Dab tsi yog qhov sib txawv tseem ceeb ntawm cov tawv nqaij sib txuas thiab cov fibrous?
Muaj ntau hom ntaub so ntswg sib txuas. Ib hom yog cov ntaub so ntswg sib txuas ua ke, tseem hu ua cov ntaub so ntswg sib txuas. Lub ntsiab lus fibrous thiab qhov sib txawv tuaj vim tias cov ntaub so ntswg sib txuas tau ua los ntawm cov fibers. Cov fibers feem ntau yog ua los ntawm collagen ntxiv rau qee qhov fibroblasts
Cov protein twg hauv qab no ua lub luag haujlwm tseem ceeb hauv kev khi cov tshuaj protein protein?
Albumin thiab al acid glycoprotein yog qhov tseem ceeb tshaj plaws thauj cov protein hauv cov ntshav. Albumin muaj cov chaw tshwj xeeb rau cov kua qaub thiab cov tshuaj yooj yim sib txuas thiab tuaj yeem cuam tshuam nrog lawv hauv cov ntshav vim tias qhov chaw thib peb raug daig tsuas yog digoxin
Cov kab mob sib koom ua ke twg yog tus cwj pwm los ntawm kev sib koom ua ke ntawm kev txav mus los thiab kev sib koom ua ke ntawm qhov hnyav ntawm cov pob qij txha uas feem ntau yog relieved los ntawm so?
Osteoarthritis feem ntau cuam tshuam rau txhais tes, qis nraub qaum, caj dab, thiab hnyav sib koom tes xws li hauv caug, lub duav, thiab ko taw