![Drabkin cov tshuaj reagent ua haujlwm li cas? Drabkin cov tshuaj reagent ua haujlwm li cas?](https://i.answers-medical.com/preview/medical-health/13904101-how-does-drabkins-reagent-work-j.webp)
Video: Drabkin cov tshuaj reagent ua haujlwm li cas?
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2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Drabkin's Reagent yog siv rau qhov muaj nuj nqis, txiav txim xim xim ntawm hemoglobin concentration hauv cov ntshav ntawm 540 nm. Drabkin tus Kev daws teeb meem cuam tshuam nrog txhua yam ntawm hemoglobin tshwj tsis yog sulfhemoglobin, cov xim uas ib txwm tshwm sim hauv ib feeb nkaus xwb hauv cov ntshav.
Ntawm no, koj ua li cas Drabkin txoj kev daws teeb meem?
Rau npaj cov Drabkin Kev daws , rov tsim dua ib lub vial ntawm Drabkin lub Reagent nrog 1000 ml dej. Tom qab ntawd ntxiv 0.5 ml ntawm 30% Brij 35 Tshuaj , Khoom Cai B 4184, rau 1000 ml ntawm kev rov tsim dua Drabkin lub Reagent . Sib tov kom zoo thiab lim yog tias cov khoom tsis haum.
Cyanmethemoglobin txoj kev yog dab tsi? Hauv Drabkin's txoj kev ntawm hemoglobin kwv yees hemoglobin yog oxidized rau methemoglobin los ntawm cov poov tshuaj ferricyanide, uas cuam tshuam nrog cyanide ions ntawm potassium cyanide los tsim cyanmethemoglobin . Cov hemoglobin tau kwv yees nrog kev pab ntawm cyanmethemoglobin nkhaus.
Zoo ib yam tuaj yeem nug, dab tsi yog qhov muaj pes tsawg leeg ntawm Drabkin cov tshuaj reagent?
Drabkin yog reagent yog siv hauv kev ntsuas hemoglobin los ntawm kev kuaj ntshav. Nws suav nrog potassium ferricyanide, potassium cyanide thiab potassium dihydrogen phosphate ua cov khoom. Potassium ferricyanide oxidizes hemoglobin rau methemoglobin thiab tom qab ntawd mus rau cyanmethemoglobin.
Lub hauv paus ntsiab lus tseem ceeb ntawm Cyanmethemoglobin txiav txim siab ntawm hemoglobin yog dab tsi?
Cyanmethemoglobin Txoj Kev txoj cai ntawm txoj kev no nyob hauv kev hloov pauv ntawm hemoglobin cov rau cyanmethemoglobin los ntawm kev ntxiv Potassium cyanide thiab ferricyanide uas nws qhov kev nqus tau ntsuas ntawm 540 nm hauv lub tshuab ntsuas hluav taws xob ntsuas hluav taws xob tiv thaiv cov txheej txheem daws teeb meem.
Pom zoo:
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Dab tsi yog qhov muaj pes tsawg leeg ntawm Drabkin's reagent?
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Drabkin's reagent yog siv los ntsuas hemoglobin los ntawm cov ntshav kuaj. Nws suav nrog potassium ferricyanide, potassium cyanide thiab potassium dihydrogen phosphate ua cov khoom. Potassium ferricyanide oxidizes hemoglobin rau methemoglobin thiab tom qab ntawd mus rau cyanmethemoglobin
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