Lub hom phiaj ntawm peptone hauv PCA media yog dab tsi?

2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44

Peptone yog kev sib xyaw ntawm cov protein thiab cov amino acids uas tau los ntawm kev rhuav tshem cov khoom ntuj xws li tsiaj nqaij, mis thiab nroj tsuag. Cov kev ua haujlwm ntawm peptone hauv cov as -ham agar yog los muab cov khoom siv protein kom cov kab mob me me tuaj yeem loj hlob.

Ib yam li ntawd, koj tuaj yeem nug, lub hom phiaj ntawm agar hauv PCA xov xwm yog dab tsi?

Phaj Suav Agar ( PCA ), tseem hu ua Standard Methods Agar (SMA), yog kev loj hlob ntawm microbiological nruab nrab feem ntau siv los ntsuas lossis tshuaj xyuas "tag nrho" lossis cov kab mob loj hlob ntawm cov qauv. Cov muaj pes tsawg leeg ntawm cov phaj agar yuav txawv, tab sis feem ntau nws muaj (w/v):

Qhov thib ob, vim li cas cov nqaij nyug rho tawm rau hauv xov xwm? Nqaij nyuj extract yog cov hmoov yaj dej uas yog siv rau lub hom phiaj dav xov xwm . Nws raug nquahu kom siv hauv kev tshuaj xyuas kab mob ntawm cov dej, mis, thiab lwm yam ntaub ntawv, qhov sib xyaw ua ke ntawm xov xwm yog qhov tseem ceeb thiab rau kev tshawb fawb txog biochemical, tshwj xeeb tshaj yog cov tshuaj tiv thaiv kab mob vim nws…

Ua kom pom qhov no, vim li cas peb thiaj ntxiv nacl rau hauv xov xwm?

Ntsev ua raws li tus neeg sawv cev xaiv thiab cuam tshuam nrog membrane permeability thiab osmotic equilibrium. Cov kab mob uas tiv taus ntsev yuav ua kom muaj kev loj hlob hnyav hauv cov kua zaub thiab ua kom khov khov tsis pub dhau 48 teev. Cov kab mob uas yog muaj peev xwm loj hlob nyob rau thaum muaj cov ntsev siab ntau yuav tseem ferment dextrose.

Dab tsi yog cov as -ham agar ua los ntawm?

Nutrient agar yog siv los ua lub hom phiaj nruab nrab rau kev loj hlob ntawm ntau yam ntawm cov kab mob uas tsis yoo mov. Nws muaj peptone, nqaij nyug extract thiab agar . Qhov no kuj yooj yim formulation muab cov cov as -ham tsim nyog rau kev rov ua dua ntawm ntau cov kab mob tsis-fastidious.