Video: Dab tsi yog txheej txheem txheej txheem?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Overlay (lub cajmeem) Overlaying yog lub cajmeem txoj kev uas tso cai rau cov program kom loj dua lub khoos phis tawj lub cim xeeb tseem ceeb. Ib qho kab ke uas ib txwm siv sib tshooj vim yog qhov txwv ntawm kev nco lub cev, uas yog lub cim xeeb sab hauv rau lub kaw lus-ntawm-nti, thiab tsis muaj cov chaw nco virtual.
Hais txog qhov no, dab tsi yog qhov tseem ceeb ntawm txheej txheem agar overlay hauv kev cais tus kab mob?
Mos Agar Overlay Cov txheej txheem: Kev tsim cov Plaques rau Muab cais nyob ib leeg thiab Enumeration of Phage (Kev Ntsuam Xyuas Plaque) Qhov no txheej txheem feem ntau yog siv los tshuaj xyuas thiab ntsuas cov kab mob bacteriophage (phage), lossis kab mob kab mob uas yog qhov loj me ntawm 100 txog 200 nm. Yuav tsum muaj lub tshuab raj tshuab hluav taws xob txhawm rau pom tus kheej ib feem ntawm phage.
Ib qho ntxiv, koj ua agar saum? Microbiology - Plating hauv Sab saum toj Agar
- Cov phaj sov sov rau chav sov ua ntej siv.
- Npaj cov agar saum toj raws li qhov tsim nyog kua nruab nrab nrog 0.7% agar.
- Qhuav sab saum toj agar hauv microwave kom tiav.
- Cia agar kom txias txog 48 °.
- Ntxiv cov cell thiab phage mus rau 13 hli daj-capped raj hauv 48 ° qhov ntsuas kub.
- Tshem lub hau thiab ntxiv 2.5 mL molten sab saum toj agar.
Ntawm no, dab tsi yog qhov ua haujlwm ntawm agar saum?
Sab saum toj agar kev npaj muaj qis dua ntawm agar (7 g/L) dua li cov kev daws teeb meem ib txwm siv los npaj agar daim hlau (15 g/L). Qhov qis agar concentration tso cai rau progeny phage los ntawm cov cell lysed kom nthuav tawm los ntawm kev tshaj xov xwm thiab kis kab mob sib kis ntawm tes.
Koj ua agar mos li cas?
Dissolve Difco Bacto- Cov Agar hauv ob qhov dej distilled (12.5 grams rau 1000 ml dej [1.25%]). Kom yaj agar , rhaub hauv ob lub rhaub dej lossis tso rau hauv lub qhov cub microwave. Dispense rau hauv cov lus yooj yim thiab ua kom tsis muaj menyuam los ntawm autoclaving. Khaws ntawm chav sov.
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