Video: Dab tsi yog qhov txawv ntawm qhov ncaj thiab tsis ncaj Elisa?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Hauv qhov ncaj elisa tsuas yog ib qho tshuaj tiv thaiv yog siv-qhov no ib qho tshuaj tiv thaiv kab mob sib txuas ncaj qha mus rau kev tshawb nrhiav enzyme. Cov indirect txoj kev xav tau ob qho tshuaj tiv thaiv kab mob-thawj cov tshuaj tiv thaiv kab mob thiab ib qho enzyme txuas nrog cov tshuaj tiv thaiv kab mob thib ob uas ntxiv rau thawj cov tshuaj tiv thaiv kab mob.
Tsuas yog li ntawd, puas yog Elisa xeem ncaj qha lossis tsis ncaj?
Thaum lub xub ntiag ntawm antigen tau tshuaj xyuas, lub npe " ncaj qha ELISA "hais txog ib qho ELISA nyob rau hauv uas tsuas yog daim ntawv lo thawj cov tshuaj tiv thaiv kab mob tau siv, thiab lo lus " indirect ELISA "hais txog ib qho ELISA nyob rau hauv uas cov antigen yog khi los ntawm thawj cov tshuaj tiv thaiv uas tom qab ntawd kuaj pom los ntawm cov ntawv sau npe thib ob antibody.
Ib tus kuj tuaj yeem nug, qhov sib txawv ntawm kev tshuaj tiv thaiv kab mob ncaj qha thiab ncaj qha yog dab tsi? Ncaj IF siv ib qho tshuaj tiv thaiv kab mob tiv thaiv lub hom phiaj ntawm kev txaus siab. Thawj qhov tshuaj tiv thaiv kab mob yog sib txuas ncaj qha mus rau fluorophore. Tsis ncaj YOG siv ob lub tshuaj tiv thaiv kab mob. Thawj cov tshuaj tiv thaiv tsis tau sib txuas thiab cov tshuaj tiv thaiv kab mob thib ob fluorophore-conjugated qhia tawm tsam thawj cov tshuaj tiv thaiv kab mob tau siv rau kev kuaj pom.
Ib qho ntxiv, Elisa tsis ncaj qha yog dab tsi?
Indirect ELISA . Tsis ncaj ELISA yog ob kauj ruam ELISA uas muaj ob txoj kev khi ntawm thawj cov tshuaj tiv thaiv kab mob thiab sau npe cov tshuaj tiv thaiv kab mob thib ob. Thawj qhov tshuaj tiv thaiv kab mob tau tsim nrog cov antigen ua raws los ntawm kev tsim tawm nrog cov tshuaj tiv thaiv kab mob thib ob. Cov qauv nrog cov tshuaj tiv thaiv yog ntxiv thiab ntxuav.
Qhov kev xeem Elisa tsis ncaj qha xeem ntawv yog dab tsi?
Hauv qhov ncaj Elisa koj tab tom kuaj pom tias muaj antigen thiab thawj qhov tshuaj tiv thaiv siv yog enzyme txuas antibody. Nyob rau hauv ib tsis ncaj ncees lawm Elisa koj tab tom kuaj cov tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob thib ob yog enzyme txuas. ntxiv cov enzyme substrate thiab tos tsib feeb.
Pom zoo:
Qhov sib txawv ntawm kev ua tus neeg sawv cev ncaj ncees thiab ua tus neeg mob siab ncaj ncees yog dab tsi?
Cov neeg mob coj ncaj ncees yog yam uas cov neeg ua haujlwm ncaj ncees tuaj yeem muaj lub luag haujlwm ncaj ncees. Tsuas yog tus neeg sawv cev ncaj ncees tuaj yeem ua haujlwm raws li tus neeg tuav lub luag haujlwm ncaj ncees rau lwm tus, thaum cov neeg mob ncaj ncees tuaj yeem yog cov khoom ntawm kev coj ncaj ncees ntawm lwm tus, tab sis tsis xav tau lawv tus kheej muaj peev xwm ua haujlwm ncaj ncees
Qhov sib txawv ntawm bilirubin ncaj qha thiab bilirubin ncaj qha yog dab tsi?
Abb. Nco tseg: Conjugated bilirubin feem ntau tsis raug hu ua 'bilirubin ncaj qha' thiab unconjugated bilirubin tsis raug hu ua 'indirect bilirubin'. Ncaj thiab ncaj qha hais txog qhov sib xyaw ntsuas lossis kuaj pom hauv kev daws teeb meem. Indirect bilirubin yog rog-soluble thiab ncaj qha bilirubin yog dej-soluble
Qhov txawv ntawm Elisa ncaj qha thiab tsis ncaj Elisa yog dab tsi?
Hauv elisa ncaj qha tsuas yog siv ib qho tshuaj tiv thaiv kab mob - qhov no ib qho tshuaj tiv thaiv kab mob sib txuas ncaj qha mus rau kev tshawb nrhiav enzyme. Elisa tsis ncaj qha xav tau ob lub tshuaj tiv thaiv kab mob-thawj cov tshuaj tiv thaiv kab mob thiab ib qho enzyme txuas nrog cov tshuaj tiv thaiv kab mob thib ob uas ntxiv rau thawj cov tshuaj tiv thaiv kab mob
Dab tsi yog qhov txawv ntawm cov khoom siv ncaj qha thiab tsis ncaj?
Hauv kev sib txuas hniav ncaj qha, tus kws kho hniav siv cov khoom siv hu ua cov khoom sib xyaw los tsim cov filling lossis inlay (hom loj dua). Cov txheej txheem tiav thaum lub sijhawm mus ntsib tom chaw ua haujlwm. Nrog rau kev kho hniav tsis zoo, tus kws kho hniav yuav siv pwm ntawm cov hniav lwj. Cov pwm no raug xa mus rau ib lub chaw kuaj mob, uas tsim ib qho kev txhaws lossis inlay
Dab tsi yog qhov txawv ntawm kev kuaj tsis ncaj thiab ncaj qha tiv thaiv kab mob?
Direct IF siv tib cov tshuaj tiv thaiv kab mob tiv thaiv lub hom phiaj ntawm kev txaus siab. Thawj cov tshuaj tiv thaiv yog ncaj qha conjugated rau fluorophore. Indirect IF siv ob lub tshuaj tiv thaiv kab mob. Thawj cov tshuaj tiv thaiv yog unconjugated thiab fluorophore-conjugated Secondary antibody qhia tawm tsam thawj cov tshuaj tiv thaiv yog siv los tshawb nrhiav