Video: Qhov txawv ntawm Elisa ncaj qha thiab tsis ncaj Elisa yog dab tsi?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Nyob rau hauv ib tug ncaj elisa tsuas yog ib qho tshuaj tiv thaiv yog siv-qhov no ib qho tshuaj tiv thaiv kab mob sib txuas ncaj qha mus rau kev tshawb nrhiav enzyme. Cov indirect txoj kev yuav tsum muaj ob lub cev tiv thaiv kab mob-thawj cov tshuaj tiv thaiv kab mob thiab ib qho enzyme txuas nrog cov tshuaj tiv thaiv kab mob thib ob uas ua tiav nrog thawj cov tshuaj tiv thaiv kab mob.
Ib yam li ntawd, tib neeg nug, qhov kev xeem Elisa ncaj qha yog dab tsi?
A ncaj qha ELISA (enzyme-txuas immunosorbent soj ntsuam ) yog lub phaj-raws li cov tshuaj tiv thaiv kab mob soj ntsuam npaj rau kev tshawb nrhiav thiab kev ntsuas ntawm ib qho kev ntsuas tshwj xeeb (xws li antigens, tshuaj tiv thaiv kab mob, cov proteins, cov tshuaj hormones, peptides, thiab lwm yam) los ntawm hauv cov qauv lom lom.
Ib yam nkaus, ob hom Elisa yog dab tsi? Plaub yam tseem ceeb ntawm ELISA yog tsis ncaj, ncaj qha, qhaub cij thiab kev sib tw.
- Direct ELISA. Cov no suav tias yog daim ntawv yooj yim tshaj plaws ntawm ELISA.
- Indirect ELISA. Tsis ncaj nraim ELISAs xav kom siv ob lub tshuaj tiv thaiv thaum lub sijhawm kuaj pom.
- Kev sib tw ELISA.
- Sandwich.
Ntawm no, dab tsi yog qhov tsis ncaj qha Elisa?
Tsis ncaj ELISA . Tsis ncaj ELISA yog ob kauj ruam ELISA uas muaj ob txoj kev khi ntawm thawj cov tshuaj tiv thaiv kab mob thiab sau npe cov tshuaj tiv thaiv kab mob thib ob. Thawj cov tshuaj tiv thaiv yog incubated nrog cov antigen tom qab incubation nrog cov tshuaj thib ob. Cov qauv nrog cov tshuaj tiv thaiv yog ntxiv thiab ntxuav.
Ob daim ntawv thov uas Elisa siv rau yog dab tsi?
Daim ntawv thov ntawm ELISA Kev txiav txim siab ntawm cov ntshav cov tshuaj tiv thaiv kab mob ntau hauv kev kuaj kab mob. Siv hauv kev lag luam zaub mov thaum kuaj pom muaj peev xwm ua xua rau zaub mov. Siv rau hauv kev kis kab mob- taug qab kev kis tus kab mob xws li. HIV, mob khaub thuas noog, nquag, khaub thuas, mob raws plab, kab mob STD thiab lwm yam.
Pom zoo:
Dab tsi yog qhov txawv ntawm qhov ncaj thiab tsis ncaj Elisa?
Hauv qhov ncaj qha elisa tsuas yog siv ib qho tshuaj tiv thaiv kab mob - qhov tshuaj tiv thaiv ib leeg yog sib txuas ncaj qha mus rau kev kuaj pom enzyme. Elisa tsis ncaj qha xav tau ob lub tshuaj tiv thaiv kab mob-thawj cov tshuaj tiv thaiv kab mob thiab ib qho enzyme txuas nrog cov tshuaj tiv thaiv kab mob thib ob uas ntxiv rau thawj cov tshuaj tiv thaiv kab mob
Qhov sib txawv ntawm bilirubin ncaj qha thiab bilirubin ncaj qha yog dab tsi?
Abb. Nco tseg: Conjugated bilirubin feem ntau tsis raug hu ua 'bilirubin ncaj qha' thiab unconjugated bilirubin tsis raug hu ua 'indirect bilirubin'. Ncaj thiab ncaj qha hais txog qhov sib xyaw ntsuas lossis kuaj pom hauv kev daws teeb meem. Indirect bilirubin yog rog-soluble thiab ncaj qha bilirubin yog dej-soluble
Dab tsi yog qhov txawv ntawm cov khoom siv ncaj qha thiab tsis ncaj?
Hauv kev sib txuas hniav ncaj qha, tus kws kho hniav siv cov khoom siv hu ua cov khoom sib xyaw los tsim cov filling lossis inlay (hom loj dua). Cov txheej txheem tiav thaum lub sijhawm mus ntsib tom chaw ua haujlwm. Nrog rau kev kho hniav tsis zoo, tus kws kho hniav yuav siv pwm ntawm cov hniav lwj. Cov pwm no raug xa mus rau ib lub chaw kuaj mob, uas tsim ib qho kev txhaws lossis inlay
Dab tsi yog qhov txawv ntawm kev kuaj tsis ncaj thiab ncaj qha tiv thaiv kab mob?
Direct IF siv tib cov tshuaj tiv thaiv kab mob tiv thaiv lub hom phiaj ntawm kev txaus siab. Thawj cov tshuaj tiv thaiv yog ncaj qha conjugated rau fluorophore. Indirect IF siv ob lub tshuaj tiv thaiv kab mob. Thawj cov tshuaj tiv thaiv yog unconjugated thiab fluorophore-conjugated Secondary antibody qhia tawm tsam thawj cov tshuaj tiv thaiv yog siv los tshawb nrhiav
Dab tsi yog qhov txawv ntawm qhov tseeb qhov pib thiab qhov sib txawv qhov pib?
Qhov pib tsis txaus yog qhov tsawg kawg ntawm kev txhawb siab xav tau rau ib tus neeg kom pom qhov kev txhawb siab 50 feem pua ntawm lub sijhawm. Qhov sib txawv ntawm qhov sib txawv yog qhov sib txawv me tshaj hauv kev txhawb nqa uas tuaj yeem kuaj pom 50 feem pua ntawm lub sijhawm