Cov txheej txheem:
Video: Koj xam cov peev txheej ntawm tes li cas?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Txhawm rau xam qhov muaj sia nyob:
- Ntxiv ua ke cov ciaj thiab tuag ntawm tes suav kom tau tag nrho ntawm tes suav.
- Faib cov nyob ntawm tes suav los ntawm tag nrho ntawm tes suav rau xam qhov feem pua muaj peev xwm .
Raws li txoj cai, koj ua li cas suav cell viability ntawm absorbance?
Absorbance kev nyeem ntawv los ntawm cov qauv xeem yuav tsum tau muab faib los ntawm cov tswj thiab muab faib ua 100 kom muab feem pua cell viability los yog kev loj hlob (saib qauv hauv qab no). Absorbance qhov tseem ceeb tshaj qhov kev tswj qhia cell proliferation , thaum qis qhov tseem ceeb qhia ntawm tes tuag los yog inhibition ntawm kev loj hlob.
Ntxiv mus, dab tsi yog qhov muaj peev xwm ntawm tes? Cell viability yog ib qho kev ntsuas ntawm qhov kev faib ua feem ntawm cov nyob, noj qab nyob zoo hlwb nyob rau hauv ib tug pej xeem. Kev muaj peev xwm ntawm tes kuj tuaj yeem ntsuas tau siv ntawm tes toxicity assays uas muab ib tug nyeem ntawm cov cim ntawm ntawm tes kev tuag, xws li poob ntawm daim nyias nyias.
Tom qab ntawd, ib tus tuaj yeem nug, koj yuav suav li cas ntawm cell viability tom qab MTT kev xeem?
Txhawm rau xam qhov muaj peev xwm ntsuas zoo li MTT, ua cov hauv qab no:
- ua qhov nruab nrab ntawm ob peb "khoob" qhov dej uas muaj koj cov tshuaj MTT tab sis * tsis muaj * hlwb.
- rho koj cov keeb kwm yav dhau los ntawm kauj ruam 1 los ntawm tag nrho cov kev ntsuas rau lub phaj no.
- suav qhov nruab nrab rau koj kev tswj hwm (= cov hlwb noj qab haus huv nrog 100% muaj peev xwm ua tau).
Dab tsi yog cell viability thiab cytotoxicity?
Cell viability thiab cytotoxicity cov tshuaj yog siv rau kev tshuaj xyuas tshuaj thiab cytotoxicity kuaj cov tshuaj. Lawv nyob ntawm ntau yam ntawm tes ua haujlwm xws li kev ua haujlwm enzyme, ntawm tes membrane permeability, ntawm tes kev ua raws, ATP kev tsim khoom, kev tsim cov co-enzyme, thiab kev ua haujlwm nucleotide.
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