Video: Vim li cas thiaj siv mos agar hauv cov quav hniav?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Plaques tsis txhob kis mus ntxiv tas mus li. Qhov nruab nrab siv hauv phage cov quav quav muaj feem pua tsawg agar thiab yog li ntawd hu ua mos agar ; nws tso cai rau kev nthuav tawm ntawm phage mus rau cov hlwb uas tsis muaj kab mob nyob ze tab sis tsis tso cai rau phages tshiab txav mus rau tej thaj chaw deb ntawm lub phaj.
Ib yam li ib tug yuav nug, yog vim li cas cov mos agar overlay siv?
Mos Agar Overlay Cov txheej txheem: Tsim cov Plaques rau kev rho tawm thiab sau npe ntawm Phage (Plaque Assay) Cov txheej txheem no feem ntau siv txhawm rau kuaj thiab ntsuas cov kab mob bacteriophage (phage), lossis cov kab mob kab mob uas muaj qhov loj ntawm 100 txog 200 nm.
Qhov thib ob, koj ua agar mos li cas? Dissolve Difco Bacto- Cov Agar hauv ob qhov dej distilled (12.5 grams rau 1000 ml dej [1.25%]). Ua kom yaj agar , rhaub hauv ob lub rhaub dej lossis tso rau hauv lub qhov cub microwave. Dispense rau hauv yooj yim aliquots thiab sterilize los ntawm autoclaving. Khaws ntawm chav tsev kub.
Ntawm no, lub hom phiaj ntawm kev ua cov tshuaj plaque yog dab tsi?
Kev Ntsuas Plaque . Cov cov quav quav tuaj yeem siv los ntxuav cov neeg clonal ntawm tus kab mob lossis txiav txim siab tus kab mob titer li cov quav hniav -forming units ib ml (pfu/ml) kom paub tias tus kab mob no yuav siv tau los kis cov hlwb thaum ua hauj lwm tom ntej. Txhua pab pawg ntawm cov kab mob sib kis tau raug xa mus ua a cov quav hniav.
Lub hom phiaj ntawm agar saum?
Sab saum toj agar kev npaj muaj qis concentration ntawm agar (7 g / L) dua li cov tshuaj ib txwm siv los npaj agar daim hlau (15 g/L). Qhov qis agar concentration tso cai rau progeny phage los ntawm cov cell lysed kom nthuav tawm los ntawm kev tshaj xov xwm thiab kis kab mob sib kis ntawm tes.
Pom zoo:
Cov kab mob hauv qhov ncauj dab tsi metabolize sucrose tsim cov nplaum glucans thiab tsim cov quav hniav?
GTF yog qhov tseem ceeb enzyme uas catalyzes sucrose rau nplaum glucans thiab pab txhawb kev tsim cov hniav plaque nyob rau hauv uas lub tsub zuj zuj ntawm metabolic acids uas ua los ntawm cov kab mob colonies ua rau lub zos demineralization ntawm cov enamel nto (Loesche, 1986)
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Yuav ua li cas yog tias cov quav hniav thaiv cov ntshav ntws hauv cov hlab plawv?
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