Video: Yuav ua li cas koj xam cov kab mob colony suav?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Xam tus naj npawb ntawm kab mob (CFU) permilliliter lossis gram ntawm cov qauv los ntawm kev faib cov naj npawb ntawm cheeb tsam los ntawm dilution factor Tus naj npawb ntawm cheeb tsam ib ml tshaj tawm yuav tsum cuam tshuam qhov tseeb ntawm cov txheej txheem thiab yuav tsum tsis txhob suav nrog ntau dua ob qhov tseem ceeb.
Raws li, koj yuav txiav txim siab pes tsawg lub nroog?
Siv cov qauv : [ Tus naj npawb ntawm colonies suav] × 10 × [how ntau lub sij hawm tus qauv yuav tsum tau sib npaug kom tau mus rau qhov qub concentration: piv txwv li, 105] = Pes tsawg pawg tsim units (CFU) permilliliter ntawm pib kab lis kev cai.
Ib yam nkaus, koj yuav suav xam cov xov tooj ntawm tes siv tau li cas? Rau xam cov viability : Yog ob leeg nyob thiab tuag suav xov tooj tau sau tseg rau txhua txheej ntawm 16 kaum squares, kwv yees muaj peev xwm tuaj yeem yog xam . Ntxiv ua ke cov ciaj thiab tuag suav xov tooj kom tau txais tag nrho suav xov tooj . Divide nyob suav xov tooj los ntawm tag nrho suav xov tooj rau xam qhov feem pua muaj peev xwm.
Kuj lo lus nug yog, koj xam xam cfu ml ntawm kab lis kev cai qub li cas?
- Txhawm rau kom paub cov naj npawb ntawm CFU / ml hauv cov qauv qub, tom qab ntawd tus lej ntawm pawg ua pawg ntawm cov phaj suav suav tau muab faib ua 1 / FDF. Qhov no coj mus rau hauv tus account tag nrho cov dilution ntawm theoriginal qauv.
- 200 CFU x 1/1/4000 = 200 CFU x 4000 = 800000 CFU/ml = 8 x 10.
- CFU/ml hauv tus qauv qub.
Tag nrho cov kab mob suav yog dab tsi?
Los ntawm Wikipedia, phau ntawv txhais lus pub dawb. Tag nrho siv tau suav (TVC), muab qhov kwv yees kwv yees ntawm kev tsom mus rau cov kab mob me me xws li kab mob , poov xab ormould spores hauv ib qho qauv. Cov suav sawv cev rau tus naj npawb ntawm colony forming units (cfu) ib g (los yog ib ml) ntawm cov qauv.
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