Cov txheej txheem:
Video: Dab tsi yog reagent siv nyob rau hauv indole xeem?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Kev xeem indole yog qhov tsim nyog txheej txheem rau kev txiav txim siab lub peev xwm ntawm cov kab mob los tsim indole los ntawm deamination ntawm tryptophan . Siv txoj hauv kev Kovacs raj, indole sib xyaw, thaum muaj a tryptophan nplua nuj nruab nrab, nrog p-Dimethylaminobenzaldehyde ntawm acid pH hauv cawv los tsim cov xim liab-violet.
Dhau li ntawd, qhov kev xeem indole xeem rau dab tsi?
Cov indole xeem yog biochemical xeem ua rau cov kab mob los txiav txim siab lub peev xwm ntawm lub cev los hloov tryptophan rau hauv indole . Qhov kev faib no tau ua los ntawm cov saw hlau ntawm ntau qhov sib txawv hauv cov enzymes, cov kab ke feem ntau hu ua "tryptophanase."
Qhov thib ob, Kovac lub reagent ua haujlwm li cas? Peb Cov tshuaj Reagent yog siv los xyuas qhov muaj indole, uas yog ib qho ntawm cov khoom kawg ntawm cov kab mob oxidation ntawm cov amino acid, tryptophan. Tryptophan yog ib qho amino acid uas tuaj yeem oxidized los ntawm qee cov kab mob los tsim peb yam khoom kawg: indole, pyruvic acid, thiab ammonia.
Yog li ntawd, vim li cas indole reagent siv los kuaj E coli?
Raws li cov kab mob loj hlob thiab sib faib, lawv tso cov enzymes tshwj xeeb rau hauv cov khoom nruab nrab. Thawj enzyme muaj nyob rau hauv tag nrho cov kab mob coliform (xws li E . Nyob rau hauv lub xub ntiag ntawm E . coli cov , qhov ntxiv ntawm Indole reagent yuav tsim ib lub nplhaib liab tshwj xeeb rau saum cov qauv.
Yuav ua li cas koj ua indole reagent?
Cov txheej txheem ntawm Indole Test
- Siv lub raj kuaj tsis huv uas muaj 4 ml ntawm tryptophan kua.
- Inoculate lub raj aseptically los ntawm kev noj cov kev loj hlob ntawm 18 mus rau 24 teev kab lis kev cai.
- Muab cov raj tso rau ntawm 37 ° C rau 24-28 teev.
- Ntxiv 0.5 ml ntawm Kovac lub reagent mus rau lub broth kab lis kev cai.
- Saib xyuas seb puas muaj lossis tsis muaj ntiv nplhaib.
Pom zoo:
Dab tsi yog qhov kev xeem hloov pauv pej xeem xeem?
Yog tus qauv dav dav ntawm cov pej xeem hloov pauv los ntawm kev yug me nyuam thiab kev tuag siab mus rau kev yug me nyuam thiab kev tuag tsawg, thiab tau pom nyob rau hauv keeb kwm ntawm ntau lub teb chaws tsim. Txoj kev xav tom qab kev hloov pauv pej xeem yog kev tsim khoom lag luam ua rau muaj kev lag luam thiab kev vam meej hauv zej zog uas tom qab ntawd cuam tshuam rau cov pej xeem kev nce nqi
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Kev ntsuam xyuas indole yog ib txoj kev zoo rau kev txiav txim siab lub peev xwm ntawm cov kab mob los tsim indole los ntawm deamination ntawm tryptophan. Siv txoj hauv kev Kovacs raj, indole sib xyaw ua ke, thaum muaj cov tryptophan nplua nuj nruab nrab, nrog p-Dimethylaminobenzaldehyde ntawm cov kua qaub pH hauv cawv kom tsim cov xim liab-violet
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