Cov txheej txheem:
Video: Dab tsi yog mounting media?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Cov nruab nrab mounting yog kev daws teeb meem uas cov hnoos qeev tau teeb tsa, feem ntau nyob hauv qab iav npog. Tej zaum nws yuav yog kua, cov pos hniav los yog cov dej tshiab, yaj hauv dej, dej cawv lossis lwm yam kuab tshuaj thiab raug kaw los ntawm huab cua sab nraud los ntawm qhov tsis hnov suab nrov. xov xwm.
Ib yam li ib tug yuav nug, dab tsi yog aqueous mounting media?
Mus tas li aqueous mounting nruab nrab yog qhov tshwj xeeb dej -raws li nruab nrab mounting tsim los rau kev khaws cia tas mus li ntawm cov ntaub so ntswg thiab cov xov tooj ntawm tes smears uas tau ua rau immunohistochemically stained nrog chromogens uas tsis tuaj yeem dehydrated nrog cov kuab tshuaj organic.
Lub hom phiaj ntawm mounting media nyob rau hauv ib qho kev sim immunofluorescence yog dab tsi? Rau qhov no lub hom phiaj a nruab nrab nruab nrab (piv txwv li Mowiol lossis Prolong Gold) yog siv los kho cov qauv ntawm lub tshuab nqus tsev vacuum thiab uas tseem tiv thaiv nws lub cev qhuav dej. Tsis tas li ntawd, mounting xov xwm nce lub refractive index, uas yog conducive rau microscopy nrog roj immersion lub hom phiaj.
Hauv qhov hais txog qhov no, koj ua li cas thiaj ua tau ib qho media mount?
Ntawm no yog ib daim ntawv qhia siv feem ntau peb nyiam:
- 20mM (qhov siab kawg) Tris, pH 8.0.
- 0.5% N-propyl gallate.
- 50-90% Glycerol.
- Tej zaum koj yuav tau sov so rau 37C thiab vortex kom nws nkag mus rau hauv cov tshuaj.
- Siv tsuas yog hais txog 6-8ul ntawm mounting media ib 18mm npog.
- Seal npog npog rau swb nrog ntsia thawv polish.
Koj yuav siv Permount nruab nrab nruab nrab li cas?
Cov lus qhia. Thov ib qho me me ntawm Permount Mounting nruab nrab mus rau qhov hnoos qeev thiab tso lub iav npog rau saum. Cov khoom no teeb tsa mus rau nyias, txheej txheej uas ua tau zoo ua cov iav npog rau qhov swb.
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