2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
- Ntsuas cov nram qab no rau hauv 1L Erlenmeyer lub hwj: 5g NaCl. 5 g ib.
- 5g tryptone ua. 5g ua.
- 2.5 g poov xab Extract. 3 g ygo.
- 7,5g ua Agar .
- ntxiv (dH2O) rau 500mL.
- Swirl los sib tov.
- Npog sab saum toj ntawm lub hwj nrog cov ntawv ci txhuas thiab daim ntawv lo nrog daim kab xev autoclave.
- Autoclave rau ntawm qhov chaw ua kua rau 20 feeb lossis raws li koj qhov autoclave qhov tshwj xeeb.
Ua raws li qhov no hauv kev txiav txim siab, koj ua li cas LB agar kanamycin daim hlau?
Tsuas ntsuas tawm 100 mg ntawm ampicillin hmoov, ntxiv rau 1 mL dej, yaj los ntawm vortexing, thiab lim kom tsis muaj menyuam. Npaj da dej ntawm 60 ℃ nrog dej txaus kom nqus dej ~ 75% ntawm lub raj mis uas muaj koj cov kua nplaum yaj.
Koj ua li cas thiaj ua tau LB phaj? Yuav ua li cas ua LB agar daim hlau
- Nqa tawm 7.5 g agar, 5 g tryptone, 5 g sodium chloride (NaCl) thiab 2.5 g poov xab extract thiab ntxiv rau 1 L Duran lub raj mis.
- Ntsuas kwv yees li 400 mL ntawm cov dej distilled thiab ntxiv rau lub raj mis Duran.
- Co lub raj mis kom yaj cov reagents.
Kuj Paub, ntau npaum li cas kanamycin koj muab tso rau ntawm LB phaj?
LB daim hlau ntxiv cov tshuaj tua kab mob 2. Kanamycin – ntxiv 1 ml ib kanamycin Tshuag (50mg / ml) ib liter ntawm agar yog li qhov kawg concentration yog 50µg / ml. Mark cov phaj nrog "K" ntawm sab.
kanamycin daim hlau kav ntev npaum li cas?
Peb cov kev paub hauv kev ua tau zoo ntawm kanamycin daim hlau yog: yog khaws cia zoo daim hlau tuaj yeem siv rau 8-10 hnub, tom qab ntawd daim hlau pib poob lawv txoj haujlwm. Tom qab txhaj tshuaj ib yuav tsum tsis txhob tos ntev tshaj 48 teev rau kev xaiv lossis Kan-R cov cheeb tsam.
Pom zoo:
Koj yuav ua li cas thiaj tau cov hlau lo tawm ntawm koj cov hniav?
Cov hlau hlau ntawm cov hniav feem ntau tuaj yeem tshem tawm los ntawm kev txhuam nrog ci dej qab zib (sodium bicarbonate) lossis tshuaj peroxide (hydrogen peroxide 3%)
Koj yuav tshem tawm cov kab nrib pleb ntawm kaum ntawm koj daim di ncauj li cas?
Koj tseem tuaj yeem siv kev kho mob hauv tsev los kho koj tus mob cheilitis, suav nrog: siv daim di ncauj ua ntu zus los tiv thaiv daim di ncauj. thov roj av jelly lossis roj txiv maj phaub rau lub qhov ncauj ntawm lub qhov ncauj, uas tuaj yeem tsim teeb meem los ntawm qaub ncaug
Koj muab kanamycin ntau npaum li cas rau ntawm daim phaj LB?
LB daim hlau ntxiv rau cov tshuaj tua kab mob 2. Kanamycin – ntxiv 1 ml kanamycin stock (50mg/ml) ib liter ntawm agar ces qhov kawg concentration yog 50µg/ml. Kos lub phaj nrog "K" ntawm sab
Puas yuav lim hlau tshem tawm cov kab mob hlau?
Cov Kab Hlau Tshem Tawm Cov Kab Mob uas txuas ntxiv ua kom tsis muaj kab mob hauv cov dej tuaj yeem pab kho kab mob ntsig txog kev xeb. Cov Hlau Lim no tswj cov tshuaj tua kab mob tsawg (xws li chlorine) hauv cov dej. Cov tshuaj tua kab mob tsawg tsawg feem ntau tsis pom, thiab yuav ua kom "kab yuaj tswj tau"
Koj ua li cas ntxuav cov hlau txhuam hlau?
Muab cov txhuam hniav txhuam hniav rau hauv cov dej xab npum, thiab txhuam cov txhuam hniav kom ntxuav cov txhuam hniav thiab tshem cov greasy residues. Txuas ntxiv txhuam cov txhuam kom txog thaum koj tau tshem tag nrho cov roj. Yaug cov txhuam kom zoo hauv dej txias thiab dai kom qhuav