Video: Koj muab kanamycin ntau npaum li cas rau ntawm daim phaj LB?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
LB daim hlau ntxiv rau cov tshuaj tua kab mob
2. Kanamycin – ntxiv 1ml ua kanamycin Tshuag (50mg / ml) ib liter ntawm agar yog li qhov kawg concentration yog 50µg / ml. Mark cov phaj nrog "K" ntawm sab.
Xav txog qhov no, koj ua LB kanamycin daim hlau li cas?
Cias ntsuas tawm 100 mg ntawm ampicillin hmoov, ntxiv rau 1 mL dej, yaj los ntawm vortexing, thiab lim kom tsis muaj menyuam. Npaj dej da dej ntawm 60 ℃ nrog dej txaus kom nqus dej ~ 75% ntawm lub raj mis uas muaj koj cov kua nplaum yaj.
Qhov thib ob, daim ntawv kanamycin nyob ntev npaum li cas? Peb qhov kev paub hauv kev ua haujlwm ntawm kanamycin daim hlau yog: yog khaws cia zoo daim hlau tuaj yeem siv rau 8-10 hnub, tom qab ntawd daim hlau pib poob lawv txoj haujlwm. Tom qab txhaj tshuaj ib yuav tsum tsis tos ntev dua 48 teev rau kev xaiv lossis Kan-R cov cheeb tsam.
Tsis tas li ntawd, koj muab ampicillin ntau npaum li cas rau ntawm LB phaj?
Npaj Cov Tshuaj Tiv Thaiv Kom Siv, dilute koj cov tshuaj tua kab mob rau hauv koj LB nruab nrab ntawm 1: 1, 000. Piv txwv li, ua kom 100 mL ntawm LB / tshuaj ampicillin kev loj hlob media, ntxiv 100 ΜL ntawm 100 mg/ml tshuaj ampicillin Tshuag (1000X Tshuag) rau 100 mL ntawm LB.
Koj siv Agar ntau npaum li cas rau daim hlau?
Qhov tsawg agar - nruab nrab mix nyob rau hauv txhua phaj , yooj yim dua lawv yuav qhuav tawm. 30 ml yog qhov zoo rau kev khaws cia ntev, 10-20 mL yog qhov zoo yog tias koj yuav mus siv cov daim hlau sai sai no.
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