Video: Vim li cas peb thiaj li xeem indole?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Indole test yog siv los txiav txim siab lub peev xwm ntawm lub cev los faib cov amino acid tryptophan los tsim cov compound indole . Tryptophan yog hydrolysed los ntawm tryptophanase los tsim peb yam khoom siv tau - ib qho uas yog yog indole . Indole xeem pab ua kom sib txawv ntawm Enterobacteriaceae thiab lwm yam tsiaj.
Tsis tas li, lub hom phiaj ntawm kev siv Kovac cov tshuaj reagent yog dab tsi?
Los ntawm Wikipedia, phau ntawv txhais lus pub dawb. Cov reagent yog biochemical reagent muaj cov isoamyl cawv, para-dimethylaminobenzaldehyde (DMAB), thiab concentrated hydrochloric acid. Nws yog siv rau kev kuaj mob indole, txhawm rau txiav txim siab lub peev xwm ntawm cov kab mob sib cais indole los ntawm cov amino acid tryptophan.
Ib tug kuj yuav nug, dab tsi cov khoom kawg kuaj pom nyob rau hauv ib tug zoo indole xeem? Lub hauv paus ntsiab lus ntawm Indole Test Tryptophanase catalyzes cov tshuaj tiv thaiv deamination, thaum lub sijhawm amine (-NH2) pawg ntawm tryptophan molecule raug tshem tawm. Cov khoom kawg ntawm cov tshuaj tiv thaiv yog indole, pyruvic acid, ammonium (NH4+) thiab lub zog.
Kuj Paub, vim li cas cov kab mob ua indole?
Kab mob tuaj yeem siv cov teeb liab molecules los tswj hwm lawv tus cwj pwm kom muaj sia nyob hauv cov zej zog sib txawv ntau yam. Indole yog dav nyob rau hauv ib puag ncig ntuj, raws li ntau yam ntawm Gram-positive thiab Gram-negative kab mob (txog hnub, 85 hom) tsim loj ntau ntawm indole.
Yuav ua li cas indole xeem?
Cov indole xeem yog txheej txheem tsim nyog los txiav txim siab lub peev xwm ntawm cov kab mob los tsim indole los ntawm deamination ntawm tryptophan. Siv txoj hauv kev Kovacs raj, indole ua ke, nyob rau hauv lub xub ntiag ntawm ib tug tryptophan nplua nuj nruab nrab, nrog p-Dimethylaminobenzaldehyde ntawm ib tug acid pH nyob rau hauv cawv los ua ib tug liab-violet compound.
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