Video: Lub hom phiaj ntawm thawj cov tshuaj tiv thaiv kab mob hauv Western blot yog dab tsi?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Nrog txoj hauv kev tshawb pom ncaj qha, ib qho enzyme- lossis fluorophore-conjugated thawj antibody yog siv los tshuaj xyuas antigen ntawm kev txaus siab ntawm blooj . Hauv txoj hauv kev tshawb nrhiav tsis ncaj, ib qho tsis muaj npe thawj antibody yog thawj zaug siv los khi rau antigen.
Ntxiv mus, dab tsi yog thawj cov tshuaj tiv thaiv kab mob hauv Western blot?
Cov thawj antibody lees paub thiab khi cov epitope lossis tshwj xeeb cov amino-acid ntu ntawm cov protein uas txaus siab. Secondary tshuaj tiv thaiv siv rau sab hnub poob blotting feem ntau yog sib txuas nrog cov enzyme; feem ntau siv cov enzymes yog Horse Radish Peroxidase (HRP) thiab Alkaline Phosphatase (AP).
Ib qho ntxiv, thawj qhov tshuaj tiv thaiv kab mob ua dab tsi? Thawj cov tshuaj tiv thaiv kab mob yog immunoglobulins uas khi rau cov antigen tshwj xeeb ( protein ntau , peptide, cov molecule me me, thiab lwm yam) ntawm kev txaus siab thiab feem ntau tsis sib xws. Thawj qhov tshuaj tiv thaiv kab mob zoo lees paub thiab khi nrog kev sib raug zoo thiab tshwj xeeb kom ua kom huv, kuaj pom, thiab ntsuas lub antigen.
Thiaj li, lub hom phiaj ntawm Western blot yog dab tsi?
A sab hnub poob yog ib txoj hauv kev sim siv txhawm rau txheeb xyuas cov protein tshwj xeeb molecules los ntawm cov sib xyaw ntawm cov protein. Ntxiv mus, cov protein molecules tau sib cais raws li lawv qhov ntau thiab tsawg siv cov txheej txheem hu ua gel electrophoresis. Tom qab kev sib cais, cov protein tau pauv los ntawm cov gel mus rau a blotting daim nyias nyias.
Cov tshuaj tiv thaiv kab mob tau siv hauv Western blotting li cas?
Antibodies yog siv txhawm rau txheeb xyuas cov hom phiaj ntawm cov protein sab hnub poob (tshuaj tiv thaiv kab mob). Cov tshuaj tiv thaiv tau sib txuas nrog cov ntawv fluorescent lossis cov ntawv hluav taws xob lossis cov enzymes uas muab cov tshuaj tiv thaiv tom ntej nrog cov tshuaj reagent, ua rau muaj xim lossis tso tawm lub teeb, ua kom pom tau.
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