Cov txheej txheem:
Video: Koj ua li cas ci ntsa iab cresyl xiav?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
1) Ntxiv 2-3 tee ntawm Ci ntsa iab cresyl xiav tov rau hauv 75-X10-mm yas raj los ntawm yas Pasteur pipette. 2) Ntxiv 2-4 ntim ntawm tus neeg mob EDTA-anticogulated ntshav rau Ci ntsa iab cresyl xiav daws thiab sib tov kom raug. 3) Khaws qhov sib tov ntawm 37 ° C rau 15-20 feeb.
Tsis tas li ntawd, dab tsi ci ntsa iab cresyl xiav stain?
Ci ntsa iab cresyl xiav yog supravital pes tsawg siv los suav cov reticulocytes. Nws tau muab cais ua xim zas xim.
Ib tus kuj yuav nug, vim li cas cov reticulocytes tsuas nrog Supravital stains? Feem ntau supravital tsuas yog ua rau reticulocytes cov siv cov xim xiav methylene tshiab lossis ci ntsa iab cresyl xiav, uas ua rau nws pom tau cov qauv reticulofilamentous ntawm ribosomes yam ntxwv ua rau muaj nag xob nag cua nyob hauv cov qe ntshav liab tsis paub qab hau los ntawm supravital stains.
Ib yam li ib tus yuav nug, koj npaj tau li cas reticulocyte stain?
Cov txheej txheem ntawm NMB stain (reticulocytes stain)
- Ntxiv ob tee ntshav rau peb tee ntawm NMB daws.
- Sib tov thiab tawm rau 15 feeb.
- Tam sim no ua peb daim nyias nyias thiab cia lawv qhuav.
- Suav tag nrho ntawm 1000 RBCs nyob rau hauv 100 x roj teeb lub lens.
- Sim ua smear nyias uas muaj nyob ib puag ncig 100 lub cell rau ib thaj tsam.
Dab tsi tshiab methylene xiav stain siv rau?
Cov ntawv thov. NMB is ib cov xim tus neeg sawv cev siv hauv kuaj mob cytopathology thiab histopathology, feem ntau yog rau cov xim cov qe ntshav liab tsis paub qab hau. Nws yog supravital pes tsawg . Nws muaj feem cuam tshuam nrog methylene xiav , ib tug laus pes tsawg hauv dav siv.
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