Vim li cas E coli tsim ntsuab ntawm EMB?
Vim li cas E coli tsim ntsuab ntawm EMB?

Video: Vim li cas E coli tsim ntsuab ntawm EMB?

Video: Vim li cas E coli tsim ntsuab ntawm EMB?
Video: Escherichia coli pathogenesis 2024, Lub Xya hli ntuj
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Nyob EMB yog E . coli cov yog zus nws yuav muab cov xim sib txawv ntsuab sheen (vim yog cov yam ntxwv metachromatic ntawm cov xim, E . coli cov txav siv flagella, thiab muaj zog acid kawg-cov khoom ntawm fermentation).

Thiaj li, vim li cas thiaj tuaj yeem siv EMB agar txhawm rau txheeb xyuas pom muaj E coli?

EMB agar tau siv hauv kev ntsuas dej kom zoo kom paub qhov sib txawv ntawm coliforms thiab fecal coliforms uas qhia tau tias muaj peev xwm ua rau cov kab mob me me sib kis tau hauv cov qauv dej ( muaj nyob ntawm E .. coli cov hauv tus dej/piv txwv dej qhia tias muaj quav ntawm cov dej sib kis puas muaj lub xub ntiag ntawm lwm yam kab mob enteric).

Ib tus kuj tseem yuav nug tau tias, cov kab mob coli zoo li cas hauv EMB? Escherichia coli tshwm sim raws li loj, xiav-dub cheeb tsam , feem ntau nrog ib tug ntsuab metallic sheen. Nyob EMB Agar (HHT), cais cheeb tsam lactose- thiab / lossis sucrose-fermenting coliforms yuav tsim xiav-dub cheeb tsam nrog cov chaw tsaus, feem ntau nrog cov xim ntsuab ntsuab. Lwm coliforms tsim mucoid, liab cheeb tsam.

Ib yam li ntawd, puas E coli loj hlob ntawm EMB agar?

Tsuas yog cov kab mob gram-negative xwb loj hlob ntawm EMB agar . Cov kab mob gram-positive raug txwv los ntawm cov dyes eosin thiab methylene blue ntxiv rau agar . Vim muaj zog fermention ntawm lactose thiab tsim tawm ntawm cov kua qaub ntau, cov cheeb tsam ntawm Escherichia coli cov pom tsaus nti thiab xiav-dub nrog cov xim ntsuab xim hlau.

Dab tsi yog xim hlau xim ntsuab?

Eosin methylene blue agar (EMB) yog ib qho kev xaiv thiab sib txawv nruab nrab siv los cais cov quav coliforms. Feem ntau cov xim daj lossis xim tsis muaj xim qhia tau hais tias lub cev tsis muaj lactose lossis sucrose thiab tsis yog quav quav. Escherichia coli feem ntau ua rau nws yog xim hlau ntsuab sheen pe EMB.

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