Cov txheej txheem:
Video: Kovac's reagent test rau dab tsi?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Cov reagent yog biochemical reagent muaj cov isoamyl cawv, para-dimethylaminobenzaldehyde (DMAB), thiab concentrated hydrochloric acid. Nws yog siv rau kev kuaj mob indole xeem , txhawm rau txiav txim siab lub peev xwm ntawm cov kab mob sib cais indole los ntawm cov amino acid tryptophan.
Cov lus nug tseem yog, qhov kev xeem indole rau dab tsi?
Cov indole xeem yog biochemical xeem ua rau cov kab mob los txiav txim siab lub peev xwm ntawm lub cev los hloov tryptophan rau hauv indole . Qhov kev faib no tau ua los ntawm cov saw hlau ntawm ntau qhov sib txawv hauv cov enzymes, cov kab ke feem ntau hu ua "tryptophanase."
Dab tsi yog Kovac reagent siv los kuaj xyuas thiab dab tsi qhia tau tias muaj txiaj ntsig zoo? Cov Cov tshuaj reagent Kovac uas koj ntxiv rau SIM nruab nrab rau xeem rau indole muaj hydrochloric acid, p-dimethylaminobenzaldehyde (DMABA), thiab n-amyl cawv. DMABA reacts nrog indole los tsim ib qho liab quinoidal compound. Yog tias reagent tig liab, qhov kev sim indole yog zoo.
Tsis tas li ntawd, Kovac lub reagent ua haujlwm li cas?
Peb Cov tshuaj Reagent yog siv los xyuas qhov muaj indole, uas yog ib qho ntawm cov khoom kawg ntawm cov kab mob oxidation ntawm cov amino acid, tryptophan. Tryptophan yog ib qho amino acid uas tuaj yeem oxidized los ntawm qee cov kab mob los tsim peb yam khoom kawg: indole, pyruvic acid, thiab ammonia.
Koj npaj li cas rau kev kuaj biochemical?
Cov Txheej Txheem Kuaj
- Dilute koj cov kab mob hauv ib lub raj ntawm cov dej tsis muaj menyuam kom tau txais cov turbidity sib npaug rau 0.5 McFarland xeem tus qauv.
- Siv lub raj mis tsis muaj menyuam 1mL, tso 1 ml ntawm kab mob rau hauv nruab nrab ntawm lub raj.
- Cap kom nruj; txhob jostle.
- Incubate rau 24 teev ntawm 37 ° C.
Pom zoo:
Dab tsi yog lub hauv paus ntawm lub cev rau amenorrhoea tom qab xeeb menyuam thiab lwm yam dab tsi uas tuaj yeem ua rau amenorrhoea?
Amenorrhea cov lus tseeb Genetic los yog yug hauv tsev yog qhov ua rau pom thawj zaug ntawm tus mob amenorrhea. Amenorrhea tuaj yeem tshwm sim los ntawm kev ua haujlwm tsis zoo ntawm zes qe menyuam, pituitary caj pas, hypothalamus, lossis lub tsev menyuam. Kev qoj ib ce hnyav, hnyav hnyav, mob lub cev, thiab kev nyuab siab tuaj yeem ua rau muaj kev tsis txaus ntseeg
Dab tsi yog qhov txiaj ntsig ib txwm rau PaO 2 Dab tsi yog qhov txiaj ntsig rau kev txiav txim siab qhov hnyav ntawm hypoxemia?
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Dab tsi ua rau tub ceev xwm saib xyuas tshuaj rau dab tsi?
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Dab tsi yog reagent siv nyob rau hauv indole xeem?
Kev ntsuam xyuas indole yog ib txoj kev zoo rau kev txiav txim siab lub peev xwm ntawm cov kab mob los tsim indole los ntawm deamination ntawm tryptophan. Siv txoj hauv kev Kovacs raj, indole sib xyaw ua ke, thaum muaj cov tryptophan nplua nuj nruab nrab, nrog p-Dimethylaminobenzaldehyde ntawm cov kua qaub pH hauv cawv kom tsim cov xim liab-violet