Video: Dab tsi yog txheej txheem tshuaj tiv thaiv kab mob fluorescent?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Ncaj Cov txheej txheem tshuaj tua kab mob fluorescent
Ncaj fluorescent antibody (DFA) kev tshuaj ntsuam siv daim ntawv lo fluorescently mAb los khi thiab ua kom pom lub hom phiaj antigen. Cov fluorescent tshuaj tiv thaiv khi rau cov kab mob ntawm lub tshuab nqus tsev twj tso kua mis, tso cai rau kev kuaj pom cov kab mob uas siv a fluorescence microscope.
Tsis tas li kom paub yog, kev tshuaj fluorescent antibody ncaj qha ua haujlwm li cas?
Cov Kev Ntsuas Tshuaj Tua Hluav Taws Xob ncaj qha kuaj pom muaj ib qho antigen (feem ntau yog ib qho protein tshwj xeeb ntawm tus kab mob, kab mob lossis lwm yam microbe). Yog tias muaj antigen, tam sim no tshuaj tiv thaiv khi los tsim ib qho tshwj xeeb heev, rhiab heev protein tag.
Ib sab saum toj no, vim li cas thiaj siv immunofluorescence? Cov txheej txheem no ua rau siv cov tshuaj fluorophores kom pom qhov chaw ntawm cov tshuaj tiv thaiv kab mob. Immunofluorescence tuaj yeem yog siv nyob rau hauv cov ntaub so ntswg, kab lis kev cai ntawm tes, los yog ib tug neeg lub hlwb, thiab tej zaum yuav siv txhawm rau txheeb xyuas kev faib cov protein, glycans, thiab cov tshuaj lom me me thiab tsis muaj tshuaj lom.
Ib tus kuj tseem yuav nug tau, dab tsi yog qhov tshuaj tiv thaiv kab mob fluorescent tiv thaiv kab mob ncaj qha?
Cov indirect fluorescent antibody test (IFA) yog ib qho khoom siv ib nrab, rhiab, thiab nrawm xeem rau kev kuaj pom tus kab mob tiv thaiv kab mob vwm (RABV) immunoglobulin M (IgM) thiab G (IgG) tshuaj tiv thaiv nyob rau hauv cov ntshav thiab cerebral spinal fluid (CSF) kuaj.
Dab tsi yog qhov sib txawv ntawm qhov ncaj thiab tsis ncaj qha immunofluorescence?
Ncaj YOG siv ib qho tshuaj tiv thaiv kab mob ncaj qha tiv thaiv lub hom phiaj ntawm kev txaus siab. Thawj cov tshuaj tiv thaiv yog ncaj qha conjugated rau fluorophore. Tsis ncaj IF siv ob lub tshuaj tiv thaiv kab mob. Thawj cov tshuaj tiv thaiv tsis tau sib txuas thiab cov tshuaj tiv thaiv kab mob thib ob fluorophore-conjugated qhia tawm tsam thawj cov tshuaj tiv thaiv kab mob tau siv rau kev kuaj pom.
Pom zoo:
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