Thermolysin cleave qhov twg?

2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44

Thermolysin nyiam cov nplooj ntawm N-terminus ntawm hydrophobic residues leucine, phenylalanine, valine, isoleucine, alanine thiab methionine. Qhov zoo ntawm kev zom zaub mov yog 65-85 ° C. Thermolysin kev ua haujlwm yog qhov zoo ntawm pH 5.0-8.5.

Tom qab ntawd, ib tus tseem tuaj yeem nug, qhov trypsin tshem tawm qhov twg?

Trypsin tau tsim hauv cov hnyuv thaum nws daim ntawv proenzyme, trypsinogen tsim los ntawm pancreas , yog qhib kom ua Trypsin cleaves peptide chains feem ntau ntawm carboxyl sab ntawm cov amino acids lysine los yog arginine. Nws yog siv rau ntau cov txheej txheem biotechnological.

Ib yam li ntawd, pepsin cleave qhov twg? Yog li, nws qhov chaw tseem ceeb ntawm kev sib txuas thiab kev ua haujlwm yog nyob rau hauv lub plab (pH 1.5 txog 2). Pepsin yuav zom txog 20% ntawm kev noj amide bonds los ntawm cleaving nyiam ntawm C- davhlau ya nyob twg ib sab ntawm cov amino acids uas muaj ntxhiab xws li phenylalanine, tryptophan, thiab tyrosine.

Ib yam li ntawd, nws tau nug, qhov twg chymotrypsin tshem tawm?

Nws siv cov tshuaj serine seem ua hydrolysis ntawm C-terminus ntawm cov ntxhiab tsw ntxhiab ntawm lwm cov protein. Chymotrypsin yog protease enzyme uas cov nplooj ntawm C-terminal phenylalanine (F), tryptophan (W), thiab tyrosine (Y) ntawm peptide chains.

Dab tsi peptide bonds trypsin paub?

Trypsin cleaves tshwj xeeb peptide daim ntawv cog lus ntawm C-terminal sab ntawm lysine thiab arginine residues, tshwj tsis yog rau -Arg-Pro- thiab -Lys-Pro- daim ntawv cog lus uas ib txwm tiv taus rau proteolysis.