Video: Dab tsi yog immunoblotting siv rau?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Western blotting (cov protein immunoblotting ) yog txheej txheem tshuaj xyuas siv txhawm rau txheeb xyuas thiab nrhiav cov proteins tshwj xeeb hauv cov qauv ntawm cov ntaub so ntswg homogenate lossis extract, raws li lawv lub peev xwm los khi rau cov tshuaj tiv thaiv tshwj xeeb.
Raws li, lub hom phiaj ntawm Western blot yog dab tsi?
A sab hnub poob yog ib txoj kev kuaj siv los kuaj xyuas cov protein molecules tshwj xeeb los ntawm kev sib xyaw ntawm cov proteins. Ntxiv mus, cov protein molecules tau sib cais raws li lawv qhov ntau thiab tsawg siv cov txheej txheem hu ua gel electrophoresis. Tom qab sib cais, cov protein tau pauv los ntawm cov gel mus rau a blotting daim nyias nyias.
Tsis tas li ntawd, qhov txawv ntawm immunoblotting thiab Western blotting yog dab tsi? Western Blotting (tseem hu ua kev tiv thaiv kab mob ) yog txheej txheem siv rau kev tshuaj xyuas tus kheej cov protein hauv a protein sib xyaw (piv txwv li cell lysate). Cov proteins ntawm no immunoblot tom qab ntawd nkag tau yooj yim rau kev tiv thaiv kab mob tiv thaiv kab mob rau kev kuaj pom. Cov tshuaj tiv thaiv yog siv los txheeb xyuas cov hom phiaj ntawm cov protein sab hnub poob ( immunoblot ).
Xav txog qhov no, cov txheej txheem immunoblotting yog dab tsi?
Cov txheej txheem Immunoblotting . Cov txheej txheem Immunoblotting siv cov tshuaj tiv thaiv kab mob (lossis lwm cov ligands tshwj xeeb hauv kev cuam tshuam cov txheej txheem ) txhawm rau txheeb xyuas cov hom phiaj ntawm cov protein ntau yam uas tsis cuam tshuam nrog. Lawv koom nrog kev txheeb xyuas cov hom phiaj protein ntawm antigen-antibody (lossis protein-ligand) cov kev xav tshwj xeeb.
Vim li cas immunoblotting txheej txheem 2 kauj ruam?
Keeb kwm, kev tshawb nrhiav txheej txheem tau ua hauv ob cov kauj ruam vim qhov txheeb ze yooj yim ntawm kev tsim cov tshuaj tiv thaiv thawj thiab theem nrab hauv kev sib cais txheej txheem . Qhov no muab cov kws tshawb fawb thiab cov tuam txhab muaj txiaj ntsig zoo hais txog kev yoog raws, thiab ntxiv kev nthuav dav kauj ruam mus nrhiav kom tau txheej txheem.
Pom zoo:
Dab tsi yog glycoprotein dab tsi yog ib qho ntawm kev siv glycoproteins?
Glycoproteins yog cov protein uas muaj cov oligosaccharide saw (glycans) covalently txuas nrog cov amino acid sab-saw. Glycoproteins kuj tseem yog ib qho tseem ceeb ua ke ntawm cov protein, uas lawv ua lub luag haujlwm hauv kev sib cuam tshuam ntawm tes -cell
Dab tsi yog qhov txiaj ntsig ib txwm rau PaO 2 Dab tsi yog qhov txiaj ntsig rau kev txiav txim siab qhov hnyav ntawm hypoxemia?
Zoo li feem ntau qhov kev kho mob ib txwm muaj txiaj ntsig thiab ntau yam, lub ntsiab lus tuaj yeem sib txawv me ntsis, tab sis feem ntau cov ntsiab lus hauv qab no siv: Ua rau me me hypoxemia: PaO2 = 60 txog 79 mmHg. Qhov nruab nrab hypoxemia: PaO2 = 40 txog 59 mmHg. Hypoxemia hnyav: PaO2 <40 mmHg
Dab tsi yog tsis siv hluav taws xob ionizing siv rau?
Cov hluav taws xob tsis-ionizing yog cov hluav taws xob qis uas tsis muaj lub zog txaus los ionize atoms lossis molecules. Nws nyob ntawm qhov qis kawg ntawm lub tshuab hluav taws xob. Cov hluav taws xob uas tsis yog-ionizing suav nrog cov kab hluav taws xob, microwaves, xov tooj cua yoj, hluav taws xob hluav taws xob, pom lub teeb thiab lasers
Dab tsi yog kev xyaum xwm txheej ceev thiab lawv siv rau dab tsi?
3510T2 Kev Kho Mob Thaum Muaj Xwm Ceev; Kev xyaum thiab kev tawm dag zog yog siv los rov xyaum cov xwm txheej xwm txheej ceev. Lawv raug tsim los muab kev cob qhia, txo kev ntxhov siab, thiab txheeb xyuas qhov txaus ntawm cov dej num thiab khoom siv thaum muaj xwm ceev
Dab tsi yog siv tus xuav rau hauv cov khoom siv thaum muaj xwm ceev?
Emergency Essentials Emergency Whistle yog ib qho tseem ceeb ntxiv rau cov khoom siv thaum muaj xwm ceev, cov khoom siv mus pw hav zoov, thiab cov cuab yeej taug kev. Siv nws los pab cawm neeg pom koj, lossis nrhiav koj pab pawg thaum taug kev lossis mus pw hav zoov. Koj yeej tsis paub thaum twg koj yuav xav tau lub suab nrov nrov thaum muaj xwm txheej ceev