Video: Acetylation ua li cas rau cov protein?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Acetylation yog ib qho kev hloov kho tseem ceeb ntawm cov protein hauv cell biology; thiab kev tshawb fawb proteomics tau txheeb pom ntau txhiab leej acetylated tsiaj txhu cov protein . Acetylation tshwm sim raws li ib tug co-translational thiab post-translational hloov ntawm cov protein , piv txwv li, histones, p53, thiab tubulins.
Tom qab ntawd, ib tug kuj yuav nug, dab tsi yog acetylation cov tshuaj tiv thaiv?
Acetylation yog tshuaj tshuaj tiv thaiv uas yog hu ua ethanoylation hauv IUPAC lub npe teev npe. Nws piav txog ib tshuaj tiv thaiv uas qhia txog pawg acetyl ua haujlwm rau hauv cov tshuaj lom neeg. Cov tshuaj sib txawv tshuaj tiv thaiv hu ua deacetylation - nws yog kev tshem tawm ntawm pawg acetyl.
Tsis tas li ntawd, qhov txawv ntawm acetylation thiab methylation yog dab tsi? Acetylation yog txheej txheem ntawm kev ntxiv ib qho acetyl pab pawg mus rau lwm lub molecule - histone lossis lwm hom protein, piv txwv li, txawm tias muaj ntau hom molecule tuaj yeem ua tau. acetylated . Methylation yog tus txheej txheem ntawm kev ntxiv ib pawg methyl rau lwm cov molecule, xws li DNA lossis histone lossis lwm yam protein.
Ib sab ntawm no, koj yuav txheeb xyuas acetylation li cas?
Ntau yam kev ntsuam xyuas tau siv los ua tiav ntes cov acetylation lossis methylation ntawm RelA. Cov kev ntsuam xyuas no suav nrog radiolabeling acetyl - lossis methyl- pawg, tiv thaiv kab mob nrog yias lossis qhov chaw tshwj xeeb acetyl - los yog methyl-lysine cov tshuaj tiv thaiv kab mob, thiab pawg spectrometry (6, 7, 16, 18, 19).
Lysine acetylation yog dab tsi?
Lysine acetylation yog ib qho protein ntau tom qab kev hloov pauv hauv cov kab mob thiab eukaryotes. Zoo ib yam li phosphorylation, lysine acetylation muaj nyob rau hauv ob qho tib si eukaryotes thiab prokaryotes thiab hloov ntau pua mus rau txhiab tus proteins hauv hlwb.
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