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Video: Dab tsi yog elution hauv chromatography?
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2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Hauv kev tshuaj xyuas thiab tshuaj lom neeg, kev tshem tawm yog cov txheej txheem ntawm rho tawm ib qho khoom los ntawm lwm tus los ntawm kev ntxuav nrog cov kuab tshuaj; raws li nyob rau hauv kev ntxuav ntawm loaded ion-exchange resins kom tshem tawm ions ntes. Predicting thiab tswj qhov kev txiav txim ntawm kev tshem tawm yog qhov tseem ceeb ntawm kab chromatographic txoj kev.
Ib yam li ib tug yuav nug, dab tsi yog qhov tseem ceeb hauv kab chromatography?
Txawb theem ( eluent ) The mobile phase or eluent yog cov kuab tshuaj lossis sib xyaw ntawm cov kuab tshuaj siv txav cov sib txuas los ntawm kem . Cov eluent yog optimized nyob rau hauv me me scale pretests, feem ntau siv nyias txheej chromatography (TLC) nrog tib theem nyob ruaj khov. Nws muaj qhov zoo tshaj plaws txaus txaus rau txhua qhov kev sib cais.
Ib tug kuj yuav nug, qhov txawv ntawm eluent thiab eluate yog dab tsi? Eluent yog ib feem ntawm theem mobile, uas nqa cov qauv ua piv txwv nrog nws. Eluate yog kev sib txuas ntawm cov theem mobile thiab cov analytes.
Tsis tas li ntawd, dab tsi yog qhov elution buffer?
Tsis tshem tawm yog ib qho hnyav hnyav hauv affinity chromatography. Elution tsis yog siv los ntxuav tawm cov proteins uas tsis muaj nyob rau thawj zaug thiab ntawm qhov ntau dua nws tso cov protein uas xav tau los ntawm ligand.
Dab tsi yog elution profile?
Qhov profile . Los ntawm Biology-Online Dictionary | Biology-online phau ntawv txhais lus. Txhais. Lub sijhawm siv cov duab nraaj ntawm cov chromatograph uas qhia pom ntau npaum li cas cov ntaub ntawv tau nqa tawm ntawm kab ntawv los ntawm cov neeg ua haujlwm zoo lossis tsis ua haujlwm nyob rau lub sijhawm.
Pom zoo:
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