B galactosidase nyob qhov twg?

Video: B galactosidase nyob qhov twg?

2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44

GLB1 noob muab cov lus qhia rau tsim cov enzyme hu ua beta - galactosidase ( β - galactosidase ). Qhov enzyme no yog nyob nyob rau hauv lysosomes, uas yog compartments nyob rau hauv lub hlwb uas zom thiab recycle ntau hom molecules.

Ntxiv mus, ß galactosidase yog dab tsi?

β - galactosidase , tseem hu ua lactase, beta -gal lub β -gal, yog glycoside hydrolase enzyme uas catalyzes hydrolysis ntawm β -galactosides rau hauv monosaccharides los ntawm kev tawg ntawm glycosidic daim ntawv cog lus. β -galactosides suav nrog cov carbohydrates uas muaj galactose qhov twg glycosidic daim ntawv cog lus nyob saum galactose molecule.

Qhov thib ob, lub hom phiaj ntawm cov noob lacZ yog dab tsi? Cov laj lacz encodes ib qho enzyme hu ua β-galactosidase, uas yog lub luag haujlwm rau faib cov lactose (disaccharide) rau hauv cov piam thaj yooj yim thiab galactose (monosaccharides). The LACY noob encodes cov protein ntau hu ua lactose permease, uas yog transmembrane "twj" uas tso cai rau lub cell xa cov lactose.

Yog li ntawd, E coli siv B galactosidase enzyme li cas?

Hauv E . coli cov , qhov no enzyme yog lub luag haujlwm tseem ceeb hauv kev tawg ntawm 12 carbon qab zib lactose rau hauv ob 6 carbohydrates suab thaj, piam thaj thiab galactose. Yog tias lactose ntxiv rau qhov nruab nrab ntawm kev loj hlob muaj qhov sib sau ua ke ntawm β - galactosidase nyob rau hauv cov kab mob.

Kev ntsuas beta galactosidase ntsuas li cas?

Rau ntsuas β - Kev ua haujlwm ntawm galactosidase Kev sib xyaw ntawm cov xim daj (nce 420 nm absorbance) / feeb yog saib xyuas.

Phosphate tsis, rau 100 ml:

1,61g ua₂HPO₄.
0.55g Nws₂PO₄.
kho pH rau 7.0.
phosphate buffer ruaj khov ntawm chav sov thiab tsis tas yuav tsum tau ua kom tshiab txhua lub sijhawm.

Pom zoo:

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