Video: Western blot siv los kuaj xyuas yog dab tsi?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Sab hnub poob . A sab hnub poob yog txoj kev sim siv los ntes cov protein molecules tshwj xeeb los ntawm kev sib xyaw ntawm cov proteins. Western blots tuaj yeem yog siv los ntsuas qhov loj ntawm cov protein uas txaus siab, thiab ntsuas tus nqi ntawm cov protein qhia.
Tsis tas li, Western blotting siv nyob qhov twg?
Western blot feem ntau yog siv hauv kev tshawb fawb cais thiab txheeb xyuas cov protein. Hauv cov txheej txheem no sib tov ntawm cov protein tau sib cais raws qhov hnyav ntawm lub cev, thiab yog li ntawm hom, los ntawm gel electrophoresis. Cov txiaj ntsig no tau xa mus rau daim nyias nyias ua cov qhab rau txhua qhov protein.
Ib tus kuj yuav nug, cov gel twg tau siv hauv Western blotting? Cov mos mos thiab nyias nyias tso cai rau hloov pauv tau cov protein . Nitrocellulose thiab polyvinylidene difluoride (PVDF) cov ntaub ntawv yog cov feem ntau siv daim nyias nyias siv rau sab hnub poob blotting mus kom ze.
Tom qab, lo lus nug yog, qhov txawv ntawm immunoblotting thiab Western blotting yog dab tsi?
Western Blotting (tseem hu ua kev tiv thaiv kab mob ) yog txheej txheem siv rau kev tshuaj xyuas tus kheej cov protein hauv a protein sib xyaw (piv txwv li cell lysate). Cov proteins ntawm no immunoblot tom qab ntawd nkag tau yooj yim rau kev tiv thaiv kab mob tiv thaiv kab mob rau kev kuaj pom. Cov tshuaj tiv thaiv yog siv los txheeb xyuas cov hom phiaj ntawm cov protein sab hnub poob ( immunoblot ).
Vim li cas Western blot zoo dua Elisa?
Western Blotting yog txoj hauv kev siv ntau tshaj plaws ntawm kev sim kom paub tseeb tias muaj txiaj ntsig zoo los ntawm ELISA xeem. Ib qho txiaj ntsig ntawm Western Blotting yog tias nws tsis tshua muaj los muab cov txiaj ntsig tsis zoo raws li nws tuaj yeem paub qhov txawv ntawm cov tshuaj tiv thaiv kab mob HIV thiab lwm yam tshuaj tiv thaiv.
Pom zoo:
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