Video: Vim li cas EMB inhibit gram positive?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
Feem ntau cov kab mob uas ferment lactose tsim cov kab mob EMB agar uas yog tsaus xiav mus rau dub nrog ib tug metallic sheen vim nag lossis daus ntawm cov dyes los ntawm cov kua qaub by-products ntawm fermentation. Kev loj hlob ntawm Gram zoo kab mob feem ntau txwv nyob rau EMB agar vim yog toxicity ntawm methlyene xiav dye.
Yog li ntawd, puas muaj kab mob Gram zoo tuaj yeem loj hlob ntawm EMB agar?
Qee hom kab mob Salmonella thiab Shigella tuaj yeem ua tsis tiav loj hlob ntawm EMB Agar . Ib txhia gram - cov kab mob zoo , xws li enterococci, staphylococci, thiab cov poov xab yuav loj hlob ntawm qhov nruab nrab no thiab feem ntau tsim cov ntsiab lus tseem ceeb. Tsis muaj kab mob, tsis muaj kab mob lactose-fermenting yuav kuj loj hlob ntawm qhov nruab nrab no.
Tsis tas li ntawd, lub hom phiaj ntawm EMB yog dab tsi? Eosin methylene xiav agar ( EMB ) yog ib qho kev xaiv thiab sib txawv nruab nrab siv los cais cov quav coliforms. Eosin Y thiab methylene xiav yog pH qhov taw qhia dyes uas ua ke los ua cov xim liab tsaus nti ntawm pH tsawg; lawv kuj pab inhibit qhov kev loj hlob ntawm feem ntau Gram zoo kab mob.
Tib neeg kuj nug, vim li cas EMB thiaj xaiv cov kab mob gram tsis zoo?
Nws yog Xaiv vim nws txhawb qee yam kab mob kom loj hlob thaum inhibiting lwm tus. Eosin Y thiab Methylene Blue feem ntau inhibits Cov kab mob gram zoo los ntawm kev loj hlob (ob peb yuav loj hlob) thiab feem ntau tso cai Gram tsis zoo kab mob kom loj hlob (ib txhia yuav tsis loj hlob). Yog tias kev loj hlob zoo, feem ntau koj muaj Cov kab mob gram tsis zoo.
Vim li cas E coli muab cov xim pleev xim rau ntawm EMB Agar?
Nyob EMB yog E . coli cov nws hlob nws yuav muab qhov txawv nws yog xim hlau ntsuab sheen (vim cov khoom metachromatic ntawm cov dyes, E . coli cov txav siv flagella, thiab muaj zog acid kawg cov khoom ntawm fermentation). Qee hom Citrobacter thiab Enterobacter tseem yuav ua li no rau EMB.
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