Video: Elisa tsis ncaj qha siv rau dab tsi?
2024 Tus sau: Michael Samuels | [email protected]. Kawg hloov kho: 2023-12-16 01:44
(https://www.abnova.com) - Lub indirect ELISA yog siv Feem ntau los txiav txim siab lub zog thiab / lossis tus nqi ntawm cov tshuaj tiv thaiv kab mob hauv ib qho qauv. Hauv qhov kev ntsuam xyuas, antigen ntawm kev txaus siab yog immobilized los ntawm kev nqus ncaj qha mus rau phaj phaj.
Hais txog qhov no, qhov tsis ncaj ncees Elisa pom dab tsi?
Tsis ncaj ELISA yog ib qho yooj yim thiab ceev txheej txheem rau paub thiab ntsuas cov tshuaj tiv thaiv kab mob lossis antigens txuas rau saum npoo tawv. Ua ib qho ntawm cov tshuaj tiv thaiv kab mob rhiab tshaj plaws, nws muaj txiaj ntsig kev lag luam hauv kev tshawb fawb hauv chaw kuaj mob, kuaj mob ntawm tus kab mob biomarkers, thiab tswj tau zoo hauv ntau yam lag luam.
Tsis tas li, Elisa ncaj qha siv rau dab tsi? A ncaj qha ELISA (enzyme-linked immunosorbent assay) yog cov tshuaj raws li cov tshuaj tiv thaiv kab mob uas tau npaj tseg rau kev tshawb nrhiav thiab kev ntsuas ntawm ib qho kev tshuaj ntsuam tshwj xeeb (piv txwv li antigens, tshuaj tiv thaiv kab mob, protein, tshuaj hormones, peptides, thiab lwm yam) los ntawm cov qauv tshuaj lom neeg nyuaj.
Yooj yim li ntawd, Elisa ncaj qha ua haujlwm li cas?
Tsis ncaj ELISA yog ob kauj ruam ELISA uas muaj ob txoj kev khi ntawm thawj cov tshuaj tiv thaiv kab mob thiab sau npe cov tshuaj tiv thaiv kab mob thib ob. Thawj cov tshuaj tiv thaiv yog incubated nrog cov antigen tom qab incubation nrog cov tshuaj thib ob. Cov qauv nrog cov tshuaj tiv thaiv yog ntxiv thiab ntxuav.
Elisa xeem yog dab tsi thiab nws ua haujlwm li cas?
Ib qho enzyme-txuas immunosorbent soj ntsuam , kuj hu ELISA los yog EIA, yog ib xeem uas kuaj thiab ntsuas cov tshuaj tiv thaiv hauv koj cov ntshav. Qhov no xeem tuaj yeem siv los txiav txim seb koj puas muaj cov tshuaj tiv thaiv kab mob ntsig txog qee yam kab mob.
Pom zoo:
Dab tsi yog qhov txawv ntawm qhov ncaj thiab tsis ncaj Elisa?
Hauv qhov ncaj qha elisa tsuas yog siv ib qho tshuaj tiv thaiv kab mob - qhov tshuaj tiv thaiv ib leeg yog sib txuas ncaj qha mus rau kev kuaj pom enzyme. Elisa tsis ncaj qha xav tau ob lub tshuaj tiv thaiv kab mob-thawj cov tshuaj tiv thaiv kab mob thiab ib qho enzyme txuas nrog cov tshuaj tiv thaiv kab mob thib ob uas ntxiv rau thawj cov tshuaj tiv thaiv kab mob
Qhov sib txawv ntawm bilirubin ncaj qha thiab bilirubin ncaj qha yog dab tsi?
Abb. Nco tseg: Conjugated bilirubin feem ntau tsis raug hu ua 'bilirubin ncaj qha' thiab unconjugated bilirubin tsis raug hu ua 'indirect bilirubin'. Ncaj thiab ncaj qha hais txog qhov sib xyaw ntsuas lossis kuaj pom hauv kev daws teeb meem. Indirect bilirubin yog rog-soluble thiab ncaj qha bilirubin yog dej-soluble
Qhov txawv ntawm Elisa ncaj qha thiab tsis ncaj Elisa yog dab tsi?
Hauv elisa ncaj qha tsuas yog siv ib qho tshuaj tiv thaiv kab mob - qhov no ib qho tshuaj tiv thaiv kab mob sib txuas ncaj qha mus rau kev tshawb nrhiav enzyme. Elisa tsis ncaj qha xav tau ob lub tshuaj tiv thaiv kab mob-thawj cov tshuaj tiv thaiv kab mob thiab ib qho enzyme txuas nrog cov tshuaj tiv thaiv kab mob thib ob uas ntxiv rau thawj cov tshuaj tiv thaiv kab mob
Dab tsi yog qhov txawv ntawm cov khoom siv ncaj qha thiab tsis ncaj?
Hauv kev sib txuas hniav ncaj qha, tus kws kho hniav siv cov khoom siv hu ua cov khoom sib xyaw los tsim cov filling lossis inlay (hom loj dua). Cov txheej txheem tiav thaum lub sijhawm mus ntsib tom chaw ua haujlwm. Nrog rau kev kho hniav tsis zoo, tus kws kho hniav yuav siv pwm ntawm cov hniav lwj. Cov pwm no raug xa mus rau ib lub chaw kuaj mob, uas tsim ib qho kev txhaws lossis inlay
Dab tsi yog qhov txawv ntawm kev kuaj tsis ncaj thiab ncaj qha tiv thaiv kab mob?
Direct IF siv tib cov tshuaj tiv thaiv kab mob tiv thaiv lub hom phiaj ntawm kev txaus siab. Thawj cov tshuaj tiv thaiv yog ncaj qha conjugated rau fluorophore. Indirect IF siv ob lub tshuaj tiv thaiv kab mob. Thawj cov tshuaj tiv thaiv yog unconjugated thiab fluorophore-conjugated Secondary antibody qhia tawm tsam thawj cov tshuaj tiv thaiv yog siv los tshawb nrhiav